Construct: ORF TRCN0000475286
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003535.1_s317c1
- Derived from:
- ccsbBroadEn_00927
- DNA Barcode:
- CACCACCAGAAGTTATCGCGGCTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LAMP1 (3916)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475286
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3916 | LAMP1 | lysosomal associated membra... | NM_005561.4 | 99.9% | 100% | 556C>A |
| 2 | human | 3916 | LAMP1 | lysosomal associated membra... | XM_011537494.2 | 95.2% | 95.2% | 1_1delAins58;3G>C;499C>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1317
- ORF length:
- 1251
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcccccggc agcgcccggc gacccctgct gctgctactg ctgttgctgc 121 tgctcggcct catgcattgt gcgtcagcag caatgtttat ggtgaaaaat ggcaacggga 181 ccgcgtgcat aatggccaac ttctctgctg ccttctcagt gaactacgac accaagagtg 241 gccctaagaa catgaccttt gacctgccat cagatgccac agtggtgctc aaccgcagct 301 cctgtggaaa agagaacact tctgacccca gtctcgtgat tgcttttgga agaggacata 361 cactcactct caatttcacg agaaatgcaa cacgttacag cgtccagctc atgagttttg 421 tttataactt gtcagacaca caccttttcc ccaatgcgag ctccaaagaa atcaagactg 481 tggaatctat aactgacatc agggcagata tagataaaaa atacagatgt gttagtggca 541 cccaggtcca catgaacaac gtgaccgtaa cgctccatga tgccaccatc caggcgtacc 601 tttccaacag cagcttcagc aggggagaga cacgctgtga acaagacagg ccttccccaa 661 ccacagcgcc ccctgcgcca cccagcccct cgccctcacc cgtgcccaag agcccctctg 721 tggacaagta caacgtgagc ggcaccaacg ggacctgcct gctggccagc atggggctgc 781 agctgaacct cacctatgag aggaaggaca acacgacggt gacaaggctt ctcaacatca 841 accccaacaa gacctcggcc agcgggagct gcggcgccca cctggtgact ctggagctgc 901 acagcgaggg caccaccgtc ctgctcttcc agttcgggat gaatgcaagt tctagccggt 961 ttttcctaca aggaatccag ttgaatacaa ttcttcctga cgccagagac cctgccttta 1021 aagctgccaa cggctccctg cgagcgctgc aggccacagt cggcaattcc tacaagtgca 1081 acgcggagga gcacgtccgt gtcacgaagg cgttttcagt caatatattc aaagtgtggg 1141 tccaggcttt caaggtggaa ggtggccagt ttggctctgt ggaggagtgt ctgctggacg 1201 agaacagcat gctgatcccc atcgctgtgg gtggtgccct GGCGGGGCTG GTCCTCATCG 1261 TCCTCATCGC CTACCTCGTC GGCAGGAAGA GGAGTCACGC AGGCTACCAG ACTATCTACC 1321 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1381 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1441 ATATATCTTG TGGAAAGGAC GACACCACCA GAAGTTATCG CGGCTGACGC GTTAAGTCga 1501 caatcaacct ctggattaca aaatttgtga aagatt