Construct: ORF TRCN0000475288
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016574.1_s317c1
- Derived from:
- ccsbBroadEn_08011
- DNA Barcode:
- CGTTACGATGTCGAATTTACTGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VPS33B (26276)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475288
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26276 | VPS33B | VPS33B late endosome and ly... | NM_018668.4 | 99.8% | 99.5% | 1540G>A;1839T>A;1843G>C |
| 2 | human | 26276 | VPS33B | VPS33B late endosome and ly... | NM_001289148.1 | 95.4% | 95.1% | (many diffs) |
| 3 | human | 26276 | VPS33B | VPS33B late endosome and ly... | NM_001289149.1 | 85% | 84.7% | (many diffs) |
| 4 | human | 26276 | VPS33B | VPS33B late endosome and ly... | XM_005254887.1 | 85% | 84.7% | (many diffs) |
| 5 | human | 26276 | VPS33B | VPS33B late endosome and ly... | XM_011521448.1 | 85% | 84.7% | (many diffs) |
| 6 | human | 26276 | VPS33B | VPS33B late endosome and ly... | XM_011521449.2 | 80.5% | 77.4% | (many diffs) |
| 7 | human | 26276 | VPS33B | VPS33B late endosome and ly... | XM_017022075.2 | 79.6% | 78% | (many diffs) |
| 8 | human | 26276 | VPS33B | VPS33B late endosome and ly... | XM_017022076.1 | 79.6% | 78% | (many diffs) |
| 9 | human | 26276 | VPS33B | VPS33B late endosome and ly... | XR_001751213.2 | 63.4% | (many diffs) | |
| 10 | mouse | 233405 | Vps33b | vacuolar protein sorting 33B | NM_178070.4 | 91.1% | 97% | (many diffs) |
| 11 | mouse | 233405 | Vps33b | vacuolar protein sorting 33B | XM_017322173.1 | 58% | 61.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1917
- ORF length:
- 1851
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ttttccccat cggccggacg cccctgagct gcctgacttc tccatgctga 121 agaggctggc tcgagaccag ctcatctatc tgctggagca gcttcctgga aaaaaggatt 181 tattcattga ggcagatctc atgagccctt tggatcgaat tgccaatgtc tccatcctga 241 agcaacacga agtagacaag ctatacaagg tggagaacaa gccagccctc agctccaatg 301 aacaattgtg cttcttggtc agaccccgca tcaagaatat gcgatacatt gccagtcttg 361 tcaatgctga caaattggct ggccgaactc gcaaatacaa agtgatcttc agccctcaaa 421 agttctatgc gtgtgagatg gtgcttgagg aagagggaat ctatggagat gtgagctgtg 481 atgaatgggc cttctctttg ctgcctcttg atgtggatct gctgagcatg gaactaccag 541 aatttttcag ggattacttt ctggaaggag atcagcgttg gatcaacact gtagctcagg 601 ccttacacct tctcagcact ctctatggac cctttccaaa ctgctatgga attggcaggt 661 gcgccaagat ggcatatgaa ttgtggagga acctggagga ggaggaggat ggcgaaacca 721 agggccgaag gccagagatt ggacatatct ttctcttgga cagagatgtg gactttgtga 781 cagcactttg ctcccaagtg gtttatgagg gcctagtaga tgacaccttc cgcatcaagt 841 gtgggagtgt cgactttggc ccagaagtca catcctctga caagagcctg aaggtgctac 901 tcaatgccga ggacaaggtg tttaatgaga ttcggaacga gcacttctcc aatgtctttg 961 gcttcttgag ccagaaggcc cggaacttgc aggcccagta tgatcgccgg agaggcatgg 1021 acattaagca gatgaagaat ttcgtgtccc aggagctcaa gggcctgaaa caggagcacc 1081 gcctgctgag tctccatatt ggggcctgtg aatccatcat gaagaagaaa accaagcagg 1141 atttccagga gctaatcaag actgagcatg cactgctaga ggggttcaac atccgggaga 1201 gcaccagcta cattgaggaa cacatagacc ggcaggtgtc gcctatagaa agcctgcgcc 1261 tcatgtgcct tttgtccatc actgagaatg gtttgatccc caaggattac cgatctctga 1321 aaacacagta tctgcagagc tatggccctg agcacctgct aaccttctcc aatctgcgaa 1381 gagctgggct cctaacggag caggcccccg gggacaccct cacagccgtg gagagtaaag 1441 tgagcaagct ggtgaccgac aaggctgcag gaaagattac tgatgccttc agttctctgg 1501 ccaagaggag caattttcgt gccatcagca aaaagctgaa tttgatccca cgtgtggacg 1561 gcgagtatga tctgaaagtg ccccgagaca tggcttacgt cttcagtggt gcttatgtgc 1621 ccctgagctg ccgaatcatt gagcaggtgc tagagcggcg aagctggcag ggCCTTGATG 1681 AGGTGGTACG GCTGCTCAAC TGCAGTGACT TTGCATTCAC AGATATGACT AAGGAAGACA 1741 AGGCTTCCAG TGAGTCCCTG CGCCTCATCT TGGTGGTGTT CTTGGGTGGT TGTACATTCT 1801 CTGAGATCTC AGCCCTCCGG TTCCTGGGCA GAGAGAAAGG CTACAGGTTC ATTTTCCTGA 1861 CGACAGCAGT CACAAACAGC GCTCGCCTTA TGGAGGCCAT GAGAGAGCTG AAAGCCTGCC 1921 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1981 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 2041 ATATATCTTG TGGAAAGGAC GACGTTACGA TGTCGAATTT ACTGGTACGC GTTAAGTCga 2101 caatcaacct ctggattaca aaatttgtga aagatt