Construct: ORF TRCN0000475299

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008709.1_s317c1
Derived from:
ccsbBroadEn_13573
DNA Barcode:
CGTCATGAGGTAACATAAGATCTA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DHRS4L2 (317749)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000475299
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 317749 DHRS4L2 dehydrogenase/reductase 4 l... NM_198083.4 98.9% 98.7% 1_6delATGCAG;55A>T
2 human 10901 DHRS4 dehydrogenase/reductase 4 NM_021004.4 80.4% 70% (many diffs)
3 human 10901 DHRS4 dehydrogenase/reductase 4 XM_006720005.3 79.6% 69% (many diffs)
4 human 317749 DHRS4L2 dehydrogenase/reductase 4 l... NM_001193635.1 72.7% 67.9% (many diffs)
5 human 10901 DHRS4 dehydrogenase/reductase 4 NM_001282987.2 71.6% 58.6% (many diffs)
6 human 10901 DHRS4 dehydrogenase/reductase 4 NM_001282988.2 68.4% 58.4% (many diffs)
7 human 10901 DHRS4 dehydrogenase/reductase 4 NM_001282989.2 67.3% 57.2% (many diffs)
8 human 10901 DHRS4 dehydrogenase/reductase 4 NM_001282990.2 66.2% 55.9% (many diffs)
9 human 728635 DHRS4L1 dehydrogenase/reductase 4 l... XM_006720246.3 65.3% 52.8% (many diffs)
10 human 317749 DHRS4L2 dehydrogenase/reductase 4 l... NM_001193636.1 56.9% 56.9% 0_1ins297
11 human 10901 DHRS4 dehydrogenase/reductase 4 XM_011536366.2 56.1% 53% (many diffs)
12 human 10901 DHRS4 dehydrogenase/reductase 4 NM_001282991.2 55% 44.2% (many diffs)
13 human 317749 DHRS4L2 dehydrogenase/reductase 4 l... NM_001193637.1 39.1% 39.1% 0_1ins297;105_106ins123
14 human 728635 DHRS4L1 dehydrogenase/reductase 4 l... NM_001277864.2 33.7% 19.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
756
ORF length:
690
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc caggctgcta ggcctctgtg cctgggcacg gaagtcggtg cggttggcca
121gctccaggat gacccgccgg gacccgctca caaataaggt ggccctggta acggcctcca
181ccgacgggat cggcttcgcc atcgcccggc gtttggccca ggacagggcc cacgtggtcg
241tcagcagccg gaagcagcag aatgtggacc aggcggtggc cacgctgcag ggggaggggc
301tgagcgtgac gggcactgtg tgccatgtgg ggaaggcgga ggaccgggag cggctggtgg
361ccatggctgt gaagcttcat ggaggtatcg atatcctagt ctccaatgct gctgtcaacc
421ctttctttgg aagcctaatg gatgtcaccg aggaggtgtg ggacaagact ctGGACATTA
481ATGTGAAGGC CCCAGCCCTG ATGACAAAGG CAGTGGTGCC AGAAATGGAG AAACGAGGAG
541GCGGCTCAGT GGTGATCGTG TCTTCCATAG CAGCCTTCAG TCCATCTCCT GGCTTCAGTC
601CTTACAATGT CAGTAAAACA GCCTTGCTGG GCCTCAACAA TACCCTGGCC ATAGAGCTGG
661CCCCAAGGAA CATTAGGGTG AACTGCCTGC ACCTGGACTT ATCAAGACTA GCTTCAGCAG
721GATGCTCTGG ATGGACAAGG AAAAAGAGGA AAGCATGCCC AACTTTCTTG TACAAAGTGG
781TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA
841CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG
901ACGTCATGAG GTAACATAAG ATCTAACGCG TTAAGTCgac aatcaacctc tggattacaa
961aatttgtgaa agatt

Download FASTA (ORF) (Full)

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