Construct: ORF TRCN0000475301
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011604.1_s317c1
- Derived from:
- ccsbBroadEn_06577
- DNA Barcode:
- CCCCCGATGGTGTATTTCTAAGGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MFAP3 (4238)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475301
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4238 | MFAP3 | microfibril associated prot... | NM_001242336.2 | 99.7% | 100% | 177T>C;549T>C;786C>T |
| 2 | human | 4238 | MFAP3 | microfibril associated prot... | NM_005927.5 | 99.7% | 100% | 177T>C;549T>C;786C>T |
| 3 | human | 4238 | MFAP3 | microfibril associated prot... | NM_001135037.2 | 59.4% | 59.6% | 0_1ins438;111T>C;348C>T |
| 4 | mouse | 216760 | Mfap3 | microfibrillar-associated p... | XM_006532851.1 | 49% | 51.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1152
- ORF length:
- 1086
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gctacattgt tgcttattca ctttagtggc aagtattatt gtgccagctg 121 cttttgtttt ggaagatgtg gacttcgacc aaatggtttc actggaagca aatcgtagtt 181 cttacaatgc atcctttccc tcaagctttg aactctcagc aagttcccac tcggatgatg 241 acgtcatcat agccaaagag ggaactagcg tttcaattga gtgtcttctc acagccagtc 301 actatgaaga tgtccattgg cacaattcaa aaggacagca actggatggc agaagcagag 361 gtggaaagtg gttggtttct gataacttcc taaacatcac caatgtagct tttgatgacc 421 gtgggctcta tacctgtttc gtcacctctc caattcgtgc ctcctactct gtcaccctac 481 gtgttatctt cacctcggga gacatgagtg tctattacat gattgtttgc ctgattgcct 541 ttacaatcac actcatcttg aatgtcacac ggctgtgcat gatgagcagc catcttcgca 601 agactgagaa ggccatcaat gagttcttta gaactgaagg ggctgagaaa cttcagaagg 661 cctttgagat tgcaaaacgt atccccatca ttacctcagc caaaactctg gagctcgcca 721 aagtcacaca atttaagacc atggagtttg ctcgttatat tgaagaactg gcaagaagtg 781 tccctcttcC ACCTCTTATT CTAAACTGTC GAGCCTTTGT TGAGGAGATG TTTGAGGCTG 841 TGCGAGTGGA TGACCCTGAT GACCTGGGTG AAAGAATTAA AGAGAGACCT GCCTTGAATG 901 CTCAAGGTGG CATCTATGTC ATTAACCCAG AGATGGGACG GAGTAATTCA CCAGGAGGAG 961 ATTCAGATGA TGGCTCTCTG AATGAACAAG GCCAGGAAAT AGCAGTTCAG GTTTCTGTCC 1021 ACCTTCAGTC AGAAACCAAA AGTATTGATA CAGAGTCTCA AGGCAGCAGT CATTTCAGTC 1081 CACCTGATGA TATAGGATCT GCAGAATCTA ACTGTAACTA CAAAGATGGG GCATATGAAA 1141 ACTGTCAGCT GTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1201 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1261 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACCC CCGATGGTGT ATTTCTAAGG 1321 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t