Construct: ORF TRCN0000475313
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014627.1_s317c1
- Derived from:
- ccsbBroadEn_13428
- DNA Barcode:
- GCTATTTCATGCATACAGCACTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FGD2 (221472)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475313
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 221472 | FGD2 | FYVE, RhoGEF and PH domain ... | XM_011514373.2 | 25.7% | 25.3% | (many diffs) |
| 2 | human | 221472 | FGD2 | FYVE, RhoGEF and PH domain ... | XM_011514372.2 | 25.6% | 25.1% | (many diffs) |
| 3 | human | 221472 | FGD2 | FYVE, RhoGEF and PH domain ... | XR_926106.1 | 22.4% | (many diffs) | |
| 4 | human | 221472 | FGD2 | FYVE, RhoGEF and PH domain ... | XR_001743248.1 | 16.7% | (many diffs) | |
| 5 | human | 221472 | FGD2 | FYVE, RhoGEF and PH domain ... | NM_173558.4 | 15.6% | 15.4% | (many diffs) |
| 6 | human | 221472 | FGD2 | FYVE, RhoGEF and PH domain ... | XM_017010429.2 | 15.5% | 15.2% | (many diffs) |
| 7 | human | 221472 | FGD2 | FYVE, RhoGEF and PH domain ... | XR_001743243.1 | 9.4% | (many diffs) | |
| 8 | human | 221472 | FGD2 | FYVE, RhoGEF and PH domain ... | XR_001743242.2 | 9.3% | (many diffs) | |
| 9 | human | 221472 | FGD2 | FYVE, RhoGEF and PH domain ... | XR_001743244.2 | 7.4% | (many diffs) | |
| 10 | human | 221472 | FGD2 | FYVE, RhoGEF and PH domain ... | XR_001743241.2 | 7.1% | (many diffs) | |
| 11 | human | 221472 | FGD2 | FYVE, RhoGEF and PH domain ... | XR_001743240.2 | 7.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 378
- ORF length:
- 312
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttGGCATGAA GGGGGCAAGT GAGGAGAAGC TGGCATCTGT GTCCAACCTG GTCACTGTGT 121 TTGAGAATAG CAGGACCCCA GAAGCAGCAC CCAGAGGCCA CAGGCTAGAG GACGTGCATC 181 ACCGCCCTGA GTGCAGGCCT CCCGAGTCCC CAGGACCACG GGAGAAGACG AATGTCGGGG 241 AGGCCGTGGG GTCTGAGCCC AGGACAGTCA GCAGGAGGTA CCTGAACTCC CTGAAGAACA 301 AGCTGTCCAG CGAAGCCTGG AGGAAATCTT GCCAGCCTGT GACCCTCTCA GGATCGGGGA 361 CGCAGGTGCC TGAGGGCTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 421 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 481 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGCTATTT CATGCATACA 541 GCACTTAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt