Construct: ORF TRCN0000475313

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014627.1_s317c1
Derived from:
ccsbBroadEn_13428
DNA Barcode:
GCTATTTCATGCATACAGCACTTA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
FGD2 (221472)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000475313
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 221472 FGD2 FYVE, RhoGEF and PH domain ... XM_011514373.2 25.7% 25.3% (many diffs)
2 human 221472 FGD2 FYVE, RhoGEF and PH domain ... XM_011514372.2 25.6% 25.1% (many diffs)
3 human 221472 FGD2 FYVE, RhoGEF and PH domain ... XR_926106.1 22.4% (many diffs)
4 human 221472 FGD2 FYVE, RhoGEF and PH domain ... XR_001743248.1 16.7% (many diffs)
5 human 221472 FGD2 FYVE, RhoGEF and PH domain ... NM_173558.4 15.6% 15.4% (many diffs)
6 human 221472 FGD2 FYVE, RhoGEF and PH domain ... XM_017010429.2 15.5% 15.2% (many diffs)
7 human 221472 FGD2 FYVE, RhoGEF and PH domain ... XR_001743243.1 9.4% (many diffs)
8 human 221472 FGD2 FYVE, RhoGEF and PH domain ... XR_001743242.2 9.3% (many diffs)
9 human 221472 FGD2 FYVE, RhoGEF and PH domain ... XR_001743244.2 7.4% (many diffs)
10 human 221472 FGD2 FYVE, RhoGEF and PH domain ... XR_001743241.2 7.1% (many diffs)
11 human 221472 FGD2 FYVE, RhoGEF and PH domain ... XR_001743240.2 7.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
378
ORF length:
312
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttGGCATGAA GGGGGCAAGT GAGGAGAAGC TGGCATCTGT GTCCAACCTG GTCACTGTGT
121TTGAGAATAG CAGGACCCCA GAAGCAGCAC CCAGAGGCCA CAGGCTAGAG GACGTGCATC
181ACCGCCCTGA GTGCAGGCCT CCCGAGTCCC CAGGACCACG GGAGAAGACG AATGTCGGGG
241AGGCCGTGGG GTCTGAGCCC AGGACAGTCA GCAGGAGGTA CCTGAACTCC CTGAAGAACA
301AGCTGTCCAG CGAAGCCTGG AGGAAATCTT GCCAGCCTGT GACCCTCTCA GGATCGGGGA
361CGCAGGTGCC TGAGGGCTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA
421TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA
481GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGCTATTT CATGCATACA
541GCACTTAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)