Construct: ORF TRCN0000475344
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016772.1_s317c1
- Derived from:
- ccsbBroadEn_04926
- DNA Barcode:
- TGCACTATGAACCCCTACCACCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- APOOL (139322)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475344
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 139322 | APOOL | apolipoprotein O like | NM_198450.6 | 100% | 100% | |
| 2 | human | 139322 | APOOL | apolipoprotein O like | XM_017029272.1 | 98.8% | 98.8% | 718_726delGGTTTGTTG |
| 3 | human | 139322 | APOOL | apolipoprotein O like | XM_011530859.2 | 97.6% | 96.3% | (many diffs) |
| 4 | human | 139322 | APOOL | apolipoprotein O like | XM_017029271.1 | 96.6% | 95.2% | (many diffs) |
| 5 | human | 139322 | APOOL | apolipoprotein O like | XM_017029273.1 | 76% | 76% | 0_1ins186;532_540delGGTTTGTTG |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 873
- ORF length:
- 804
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggccatc aggatgggaa aactgacaac catgcctgca ggtctgatat 121 atgcatctgt aagtgtacat gcagccaaac aagaggaatc caaaaagcag ctagtgaaac 181 cagagcagct ccccatatat actgcaccac cgctccagtc taaatatgtt gaagagcagc 241 ctggtcattt acaaatgggc tttgcttcca tccgcactgc aactggttgt tacattggct 301 ggtgcaaggg tgtttatgtc tttgtgaaaa atgggataat ggatacagta caatttggaa 361 aagatgctta tgtctatctg aagaatcctc ctcgagattt tcttccgaaa atgggagtta 421 ttacagtttc aggattggcg ggcttggttt cagcgagaaa aggttccaag tttaagaaaa 481 ttacttatcc tctgggactg gccactttag gagcaactgt ttgctaccca gttcagtccg 541 taataattgc TAAGGTAACA GCAAAAAAGG TATATGCTAC AAGCCAGCAA ATTTTTGGAG 601 CAGTTAAATC ATTGTGGACA AAAAGCAGCA AAGAAGAGTC ACTCCCTAAA CCTAAAGAAA 661 AAACTAAGCT AGGATCCTCT TCCGAAATAG AAGTACCTGC AAAAACAACT CACGTCTTGA 721 AACACTCAGT GCCCTTGCCA ACAGAACTCA GCTCTGAAGC AAAGACCAAA TCAGAATCCA 781 CTTCAGGTGC AACCCAGTTT ATGCCTGACC CCAAGCTCAT GGATCACGGG CAGTCCCACC 841 CAGAAGATAT AGATATGTAC AGCACTAGAA GCTTGCCAAC TTTCTTGTAC AAAGTGGTTG 901 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 961 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATG 1021 CACTATGAAC CCCTACCACC TTACGCGTTA AGTCgacaat caacctctgg attacaaaat 1081 ttgtgaaaga tt