Construct: ORF TRCN0000475346
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010150.1_s317c1
- Derived from:
- ccsbBroadEn_10765
- DNA Barcode:
- TACTGACAAACGCCTATGCAGTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CYP19A1 (1588)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475346
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1588 | CYP19A1 | cytochrome P450 family 19 s... | NM_000103.4 | 43% | 41.7% | (many diffs) |
| 2 | human | 1588 | CYP19A1 | cytochrome P450 family 19 s... | NM_001347248.1 | 43% | 41.7% | (many diffs) |
| 3 | human | 1588 | CYP19A1 | cytochrome P450 family 19 s... | NM_001347249.2 | 43% | 41.7% | (many diffs) |
| 4 | human | 1588 | CYP19A1 | cytochrome P450 family 19 s... | NM_001347250.1 | 43% | 41.7% | (many diffs) |
| 5 | human | 1588 | CYP19A1 | cytochrome P450 family 19 s... | NM_001347251.1 | 43% | 41.7% | (many diffs) |
| 6 | human | 1588 | CYP19A1 | cytochrome P450 family 19 s... | NM_001347252.1 | 43% | 41.7% | (many diffs) |
| 7 | human | 1588 | CYP19A1 | cytochrome P450 family 19 s... | NM_001347253.1 | 43% | 41.7% | (many diffs) |
| 8 | human | 1588 | CYP19A1 | cytochrome P450 family 19 s... | NM_001347254.1 | 43% | 41.7% | (many diffs) |
| 9 | human | 1588 | CYP19A1 | cytochrome P450 family 19 s... | NM_001347255.2 | 43% | 41.7% | (many diffs) |
| 10 | human | 1588 | CYP19A1 | cytochrome P450 family 19 s... | NM_001347256.2 | 43% | 41.7% | (many diffs) |
| 11 | human | 1588 | CYP19A1 | cytochrome P450 family 19 s... | NM_031226.3 | 43% | 41.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 720
- ORF length:
- 654
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt tttggaaatg ctgaacccga tacattataa catcaccagc atcgtgcctg 121 aagccatgcc tgctgccacc atgccagtcc tgctcctcac tggccttttt ctcttggtgt 181 ggaattatga gggcacatcc tcaataccag gtcctggcta ctgcatggga attggacccc 241 TCATCTCCCA CGGCAGATTC CTGTGGATGG GGATCGGCAG TGCCTGCAAC TACTACAACC 301 GGGTATATGG AGAATTCATG CGAGTCTGGA TCTCTGGAGA GGAAACACTC ATTATCAGCA 361 AGTCCTCAAG TATGTTCCAC ATAATGAAGC ACAATCATTA CAGCTCTCGA TTCGGCAGCA 421 AACTTGGGCT GCAGTGCATC GGTATGCATG AGAAAGGCAT CATATTTAAC AACAATCCAG 481 AGCTCTGGAA AACAACTCGA CCCTTCTTTA TGAAAGCTCT GTCAGGCCCC GGCCTTGTTC 541 GTATGGTCAC AGTCTGTGCT GAATCCCTCA AAACACATCT GGACAGGTTG GAGGAGGTGA 601 CCAATGAATC GGGCTATGTG GACGTGTTGA CCCTTCTGCG TCGTGTCATG CTGGACACCT 661 CTAACACGCT CTTCTTGAGG ATCCCTTTGG ACGGTACTGA AATTTTCACT CTCACATCTT 721 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 781 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 841 TTTATATATC TTGTGGAAAG GACGATACTG ACAAACGCCT ATGCAGTTCA CGCGTTAAGT 901 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt