Construct: ORF TRCN0000475365
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010590.1_s317c1
- Derived from:
- ccsbBroadEn_09477
- DNA Barcode:
- TACCCTGCGACCATACCCGATCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SENP8 (123228)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475365
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 123228 | SENP8 | SUMO peptidase family membe... | NM_001166340.1 | 99.8% | 99.5% | 619A>G |
| 2 | human | 123228 | SENP8 | SUMO peptidase family membe... | NM_001172109.1 | 99.8% | 99.5% | 619A>G |
| 3 | human | 123228 | SENP8 | SUMO peptidase family membe... | NM_001172110.1 | 99.8% | 99.5% | 619A>G |
| 4 | human | 123228 | SENP8 | SUMO peptidase family membe... | NM_001172111.2 | 99.8% | 99.5% | 619A>G |
| 5 | human | 123228 | SENP8 | SUMO peptidase family membe... | NM_145204.4 | 99.8% | 99.5% | 619A>G |
| 6 | human | 123228 | SENP8 | SUMO peptidase family membe... | XM_005254157.3 | 99.8% | 99.5% | 619A>G |
| 7 | human | 123228 | SENP8 | SUMO peptidase family membe... | XM_011521217.2 | 99.8% | 99.5% | 619A>G |
| 8 | human | 123228 | SENP8 | SUMO peptidase family membe... | XM_011521215.2 | 82.6% | 82.4% | 1_132del;751A>G |
| 9 | mouse | 71599 | Senp8 | SUMO/sentrin specific pepti... | NM_001172068.1 | 86.7% | 89.1% | (many diffs) |
| 10 | mouse | 71599 | Senp8 | SUMO/sentrin specific pepti... | NM_001172069.1 | 86.7% | 89.1% | (many diffs) |
| 11 | mouse | 71599 | Senp8 | SUMO/sentrin specific pepti... | NM_001172070.1 | 86.7% | 89.1% | (many diffs) |
| 12 | mouse | 71599 | Senp8 | SUMO/sentrin specific pepti... | NM_027838.3 | 86.7% | 89.1% | (many diffs) |
| 13 | mouse | 71599 | Senp8 | SUMO/sentrin specific pepti... | NM_001172071.1 | 81.9% | 84.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 702
- ORF length:
- 636
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ccccgtagtc ttgagttaca tggacagtct actgcggcaa tcagatgtct 121 cactattgga tccgccaagc tggctcaatg accatattat tgggtttgcg tttgagtact 181 ttgccaacag tcagtttcat gactgctctg atcacgtcag tttcatcagc cctgaagtca 241 cccagttcat caagtgcact agcaacccag cagagattgc catgttcctt gaaccactgg 301 aCCTCCCCAA CAAGAGAGTT GTATTTTTAG CCATCAATGA TAACTCCAAC CAGGCAGCTG 361 GAGGAACCCA CTGGAGTTTA TTGGTCTACC TCCAAGATAA AAATAGCTTT TTTCATTATG 421 ATTCCCATAG CAGGAGCAAC TCAGTTCACG CAAAGCAGGT AGCAGAGAAA CTGGAGGCTT 481 TCTTAGGCAG AAAAGGAGAC AAACTGGCCT TTGTGGAAGA GAAAGCCCCT GCCCAACAAA 541 ACAGCTATGA CTGTGGGATG TACGTGATAT GTAACACTGA GGCCTTGTGT CAGAACTTCT 601 TTAGGCAACA GACAGAATCA CTGCTGCAGC TACTCACCCC TGCATACATC ACAAAGAAGA 661 GGGGAGAATG GAAAGATCTC ATTGCCACAC TTGCTAAAAA GTACCCAACT TTCTTGTACA 721 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 781 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 841 AGGACGATAC CCTGCGACCA TACCCGATCT CACGCGTTAA GTCgacaatc aacctctgga 901 ttacaaaatt tgtgaaagat t