Construct: ORF TRCN0000475372
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007564.1_s317c1
- Derived from:
- ccsbBroadEn_13711
- DNA Barcode:
- GATGCAATGCTAAGATCTATCTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BOLA2 (552900)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475372
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 552900 | BOLA2 | bolA family member 2 | NM_001320579.1 | 81.3% | 70.6% | (many diffs) |
| 2 | human | 552900 | BOLA2 | bolA family member 2 | NM_001031827.2 | 80.9% | 78.9% | (many diffs) |
| 3 | human | 107282092 | BOLA2-SMG1P6 | BOLA2-SMG1P6 readthrough | NM_001320623.1 | 43.3% | 42% | (many diffs) |
| 4 | human | 654483 | BOLA2B | bolA family member 2B | NM_001039182.4 | 37.5% | 35.5% | (many diffs) |
| 5 | human | 107282092 | BOLA2-SMG1P6 | BOLA2-SMG1P6 readthrough | NM_001320622.1 | 35% | 31.4% | 260_261ins38;335_915del |
| 6 | human | 107282092 | BOLA2-SMG1P6 | BOLA2-SMG1P6 readthrough | NM_001320624.1 | 34.5% | 31.8% | (many diffs) |
| 7 | human | 654483 | BOLA2B | bolA family member 2B | NM_001320607.2 | 32.6% | 21% | (many diffs) |
| 8 | human | 107282092 | BOLA2-SMG1P6 | BOLA2-SMG1P6 readthrough | NR_135316.1 | 20.9% | (many diffs) | |
| 9 | mouse | 66162 | Bola2 | bolA-like 2 (E. coli) | NM_175103.3 | 33.4% | 32.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 93
- ORF end:
- 465
- ORF length:
- 372
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA CCATGGCAAG CGCGAAAAGC CTGGACCGCT 121 GGAAAGCCCG GCTGCTTGAG GGCGGAAGTA CTGCGTTGAC GTACGCCTTA GTAAGGGCGG 181 AAGTGAGTTT TCCAGCGGAA GTGGCTCCTG TAAGGCAGCA AGGTAGCGTG GCCGGCGCCC 241 GAGCTGGGGT TGTGTCCCTG CTGGGCTGCC GTTCCAGCTG GACTGCCGCC ATGGAACTCA 301 GCGCCGAATA CCTCCGCGAG AAGCTGCAGC GGGACCTGGA GGCGGAGCAT GTGGAGGTGG 361 AGGACACGAC CCTCAACCGT TGCTCCTGTA GCTTCCGAGT CCTGGTGGTG TCGGCCAAGT 421 TCGAGGGGAA ACCGCTGCTT CAGAGACACA GGTTCTGTAC AGAGTTGCCA ACTTTCTTGT 481 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 541 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 601 GAAAGGACGA GATGCAATGC TAAGATCTAT CTCAACGCGT TAAGTCgaca atcaacctct 661 ggattacaaa atttgtgaaa gatt