Construct: ORF TRCN0000475387
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006221.1_s317c1
- Derived from:
- ccsbBroadEn_03735
- DNA Barcode:
- TCGCTGTCTTGCGATACCTTTGAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KCNK13 (56659)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475387
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56659 | KCNK13 | potassium two pore domain c... | NM_022054.4 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1290
- ORF length:
- 1224
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tggccggggt ttcagctggg gcccgggcca cctgaacgag gacaacgcgc 121 gctttctgct gctggccgcg ctcatcgtgc tctacctgct gggcggcgcc gccgtcttct 181 ccgcgctgga gctggcgcac gagcgccagg ccaagcagcg ctgggaggag cgcctggcca 241 acttcagccg cggccacaac ctgagccgcg acgagctgcg cggcttcctc cgccactacg 301 aggaggccac tcgggccggc atccgcgtgg acaacgtccg cccgcgctgg gacttcaccg 361 gcgccttcta cttcgtgggc accgtcgttt ccaccatagg gtttgggatg acaactccgg 421 cgacagtagg aggaaaaatc tttctgatct tttacggcct tgttgggtgt tccagcacca 481 tcttgttctt caacctcttc ctggagcgcc tgatcaccat catcgcctac atcatgaagt 541 cgtgccacca gcggcagctc cggagacgag gggccctgcc ccaggagagc ctgaaggatg 601 cggggcagtg tgaggtggac agcctggccg gctggaagcc ctccgtgtac tacgtcatgc 661 tgatcctatg cacagcctcc atcctcatct cttgctgcgc ctcagccatg tacaccccca 721 ttgaaggctg gagctacttt gactcactct acttctgttt tgtggctttc agcaccattg 781 gctttgggga cctggtcagc agccagaacg cccactatga gagccaaggc ctctatcgct 841 ttgccaactt cgtcttcatc ctcatgggtg tctgctgcat ctactccttg ttcaatgtca 901 tctctatcct catcaaacag tccttgaact ggatccTGAG GAAAATGGAC AGCGGGTGCT 961 GCCCGCAATG CCAGAGAGGA CTCTTGCGAT CACGCAGGAA CGTGGTGATG CCAGGCAGCG 1021 TCCGGAACCG CTGCAACATC TCCATAGAGA CAGACGGGGT GGCAGAGAGT GACACGGACG 1081 GGCGCCGGCT CTCAGGGGAG ATGATCTCCA TGAAGGACTT GCTGGCAGCC AACAAGGCCT 1141 CGTTGGCCAT CCTGCAGAAG CAACTGTCTG AGATGGCCAA CGGCTGCCCC CACCAGACCA 1201 GCACACTGGC CCGGGACAAT GAATTCTCAG GGGGGGTGGG AGCCTTTGCA ATCATGAACA 1261 ACAGGTTGGC AGAGACCAGT GGGGACAGGT ACCCAACTTT CTTGTACAAA GTGGTTGATA 1321 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1381 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATCGCT 1441 GTCTTGCGAT ACCTTTGAGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1501 tgaaagatt