Construct: ORF TRCN0000475404

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007993.1_s317c1
Derived from:
ccsbBroadEn_13638
DNA Barcode:
TTCAGCACCTCATCCATCCTTGAG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
LRRC10 (376132)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000475404
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 376132 LRRC10 leucine rich repeat contain... NM_201550.4 90.2% 90.2% 1_81del
2 mouse 237560 Lrrc10 leucine rich repeat contain... NM_146242.2 77.3% 79.4% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
819
ORF length:
750
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gccgctggac aagatggtgg atctgagtgg gagccagtta cgccgcttcc
121ccctgcacgt gtgctccttc agggagctgg tcaagctcta cctgagcgac aaccacctca
181atagcctgcc tccggagctg gggcagctac agaacctgca gattctggcc ttggatttca
241acaacttcaa ggctctgccc caggtggtgt gcaccttgaa acagctctgc atcctctacc
301tgggcaacaa caaactctgc gacctcccca gtgagctgag cctgctccag aacctcagga
361ccctgtggat cgaggccaac tgcctcaccc agctgccgga tgtggtctgt gagctgagtc
421tccttaagac tctgcatgcc ggctccaacg ccctgcgttt gctgccaggc cagctccggc
481gcctccagga gctgaggacc atctGGCTCT CGGGCAACCG GCTAACTGAC TTTCCCACTG
541TGCTGCTTCA CATGCCCTTC CTGGAGGTGA TTGATGTGGA CTGGAACAGC ATCCGTTACT
601TCCCCAGCCT GGCGCACCTG TCAAGTCTGA AGCTGGTCAT CTATGACCAC AATCCTTGCA
661GGAACGCACC CAAGGTGGCC AAAGGTGTGC GCCGTGTGGG GAGATGGGCA GAGGAGACGC
721CAGAGCCCGA CCCTAGAAAA GCCAGGCGCT ATGCGTTGGT CAGAGAGGAA AGCCAGGAGC
781TACAGGCACC AGTCCCTCTA CTTCCTCCTA CCAACTCCTT GCCAACTTTC TTGTACAAAG
841TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT
901GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG
961ACGATTCAGC ACCTCATCCA TCCTTGAGAC GCGTTAAGTC gacaatcaac ctctggatta
1021caaaatttgt gaaagatt

Download FASTA (ORF) (Full)