Construct: ORF TRCN0000475405
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010489.1_s317c1
- Derived from:
- ccsbBroadEn_15648
- DNA Barcode:
- TGGGATTCTTTATGCGCGAATGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EIF2S2 (8894)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475405
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8894 | EIF2S2 | eukaryotic translation init... | XM_017028118.1 | 65.2% | 65.2% | 1_342del |
| 2 | human | 8894 | EIF2S2 | eukaryotic translation init... | NM_001316363.2 | 64.8% | 64.8% | 1_348del |
| 3 | human | 8894 | EIF2S2 | eukaryotic translation init... | NM_003908.5 | 64.2% | 64.2% | 1_357del |
| 4 | human | 8894 | EIF2S2 | eukaryotic translation init... | NM_001316364.2 | 58.5% | 58.5% | 1_357del;683_684ins57 |
| 5 | mouse | 67204 | Eif2s2 | eukaryotic translation init... | NM_026030.2 | 59.9% | 62.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 708
- ORF length:
- 642
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct tggcaataaa aagaagaaaa agaagaatgt taagttccca gatgaggatg 121 aaatactaga gaaagatgaa gctctagaag atgaagacaa caaaaaagat gatggtatct 181 cattcagtaa tcagacaggc cctgcttggg caggcTCAGA AAGAGACTAC ACATACGAGG 241 AGCTGCTGAA TCGAGTGTTC AACATCATGA GGGAAAAGAA TCCAGATATG GTTGCTGGGG 301 AGAAAAGGAA ATTTGTCATG AAACCTCCAC AAGTCGTCCG AGTAGGAACC AAGAAAACTT 361 CTTTTGTCAA CTTTACAGAT ATCTGTAAAC TATTACATCG TCAGCCCAAA CATCTCCTTG 421 CATTTTTGTT GGCTGAATTG GGTACAAGTG GTTCTATAGA TGGTAATAAC CAACTTGTAA 481 TCAAAGGAAG ATTCCAACAG AAACAGATAG AAAATGTCTT GAGAAGATAT ATCAAGGAAT 541 ATGTCACTTG TCACACATGC CGATCACCGG ACACAATCCT GCAGAAGGAC ACACGACTCT 601 ATTTCCTACA GTGCGAAACT TGTCATTCTA GATGTTCTGT TGCCAGTATC AAAACCGGCT 661 TCCAGGCTGT CACGGGCAAG CGAGCACAGC TCCGTGCCAA AGCTAACTAC CCAACTTTCT 721 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 781 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 841 GTGGAAAGGA CGATGGGATT CTTTATGCGC GAATGCCACG CGTTAAGTCg acaatcaacc 901 tctggattac aaaatttgtg aaagatt