Construct: ORF TRCN0000475423
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015619.1_s317c1
- Derived from:
- ccsbBroadEn_00530
- DNA Barcode:
- ACTACCGAGTTATGGTGGCGTCAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- F8 (2157)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475423
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2157 | F8 | coagulation factor VIII | NM_019863.2 | 100% | 100% | |
| 2 | human | 2157 | F8 | coagulation factor VIII | NM_000132.3 | 9% | 8.9% | (many diffs) |
| 3 | mouse | 14069 | F8 | coagulation factor VIII | NM_001161373.1 | 8.2% | 8% | (many diffs) |
| 4 | mouse | 14069 | F8 | coagulation factor VIII | XM_006527791.4 | 8.2% | 7.9% | (many diffs) |
| 5 | mouse | 14069 | F8 | coagulation factor VIII | XM_006527792.4 | 8.2% | 7.9% | (many diffs) |
| 6 | mouse | 14069 | F8 | coagulation factor VIII | NM_001161374.1 | 8.2% | 7.9% | (many diffs) |
| 7 | mouse | 14069 | F8 | coagulation factor VIII | XM_006527790.4 | 8% | 7.8% | (many diffs) |
| 8 | mouse | 14069 | F8 | coagulation factor VIII | NM_007977.2 | 8% | 7.7% | (many diffs) |
| 9 | mouse | 14069 | F8 | coagulation factor VIII | XM_006527789.4 | 7.9% | 7.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 714
- ORF length:
- 648
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcg gatccaagac cctgggaagg tcttctttgg caatgtggat tcatctggga 121 taaaacacaa tatttttaac cctccaatta ttgctcgata catccgtttg cacccaactc 181 attatagcat tcgcagcact cttcgcatgg agttgatggg ctgtgattta aatagttgca 241 gcatgccatt gggaatggag agtaaagcaa tatcagatgc acagattact gcttcatcct 301 actttaccaa tatgtttgcc acctggtctc cttcaaaagc tcgacttcac cTCCAAGGGA 361 GGAGTAATGC CTGGAGACCT CAGGTGAATA ATCCAAAAGA GTGGCTGCAA GTGGACTTCC 421 AGAAGACAAT GAAAGTCACA GGAGTAACTA CTCAGGGAGT AAAATCTCTG CTTACCAGCA 481 TGTATGTGAA GGAGTTCCTC ATCTCCAGCA GTCAAGATGG CCATCAGTGG ACTCTCTTTT 541 TTCAGAATGG CAAAGTAAAG GTTTTTCAGG GAAATCAAGA CTCCTTCACA CCTGTGGTGA 601 ACTCTCTAGA CCCACCGTTA CTGACTCGCT ACCTTCGAAT TCACCCCCAG AGTTGGGTGC 661 ACCAGATTGC CCTGAGGATG GAGGTTCTGG GCTGCGAGGC ACAGGACCTC TACTGCCCAA 721 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 781 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 841 TATCTTGTGG AAAGGACGAA CTACCGAGTT ATGGTGGCGT CACACGCGTT AAGTCgacaa 901 tcaacctctg gattacaaaa tttgtgaaag att