Construct: ORF TRCN0000475429
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001338.1_s317c1
- Derived from:
- ccsbBroadEn_10915
- DNA Barcode:
- ACCGGCATCGATTTAACCTCCATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IGL (3535)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475429
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 100423062 | IGLL5 | immunoglobulin lambda like ... | NM_001256296.2 | 52.1% | 43.2% | (many diffs) |
| 2 | human | 723778 | MIR650 | microRNA 650 | NR_030755.1 | 6.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 774
- ORF length:
- 708
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ctgggctctg ctcctcctca gcctcctcac tcagggcaca ggatcctggg 121 ctcagtctgc cctgactcag cctcgctcag tgtccgggtc tcctggacag tcagtcacca 181 tcccctgcac tggaaccagc agtgatgttg gtaattataa ctatgtctcc tggtaccgac 241 aacacccagg caaagccccc aaactcatga tttatgatgt caataagcgg ccctcagggg 301 tccctgatcg cttctctggc tccaagtctg gcaacacggc ctccctgacc atctctgggc 361 tccaggctga ggatgaggct gattattact gctgctcata tgcaggcacc tacactttcg 421 gggtgttcgg cggagggacc aagctgaccg tcctaggtca gcccaaggct gccccctcgg 481 tcactctgtt cccaccctcc tctgaggagc ttcaagccaa caaggccaca ctggtgtgtc 541 tcataagtga cttctacccg GGAGCCGTGA CAGTGGCCTG GAAGGCAGAT AGCAGCCCCG 601 TCAAGGCGGG AGTGGAGACC ACCACACCCT CCAAACAAAG CAACAACAAG TACGCGGCCA 661 GCAGCTACCT GAGCCTGACG CCTGAGCAGT GGAAGTCCCA CAAAAGCTAC AGCTGCCAGG 721 TCACGCATGA AGGGAGCACC GTGGAGAAGA CAGTGGCCCC TACAGAATGT TCATACCCAA 781 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 841 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 901 TATCTTGTGG AAAGGACGAA CCGGCATCGA TTTAACCTCC ATTACGCGTT AAGTCgacaa 961 tcaacctctg gattacaaaa tttgtgaaag att