Construct: ORF TRCN0000475437
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002623.1_s317c1
- Derived from:
- ccsbBroadEn_06392
- DNA Barcode:
- AGGGAGCTTCATAGAACTAACTCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HOXB7 (3217)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475437
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3217 | HOXB7 | homeobox B7 | NM_004502.4 | 99.8% | 99.5% | 25A>G |
| 2 | mouse | 15415 | Hoxb7 | homeobox B7 | NM_010460.2 | 93.5% | 94.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 717
- ORF length:
- 651
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag ttcattgtat tatgcgaatg ctttattttc taaatatcca gcctcaagtt 121 cggttttcgc taccggagcc ttcccagaac aaacttcttg tgcgtttgct tccaaccccc 181 agcgcccggg ctatggagcg ggttcgggcg cttccttcgc cgcctcgatg cagggcttgt 241 accccggcgg ggggggcatg gcgggccaga gcgcggccgg cgtctacgcg gCCGGCTATG 301 GGCTCGAGCC GAGTTCCTTC AACATGCACT GCGCGCCCTT TGAGCAGAAC CTCTCCGGGG 361 TGTGTCCCGG CGACTCCGCC AAGGCGGCGG GCGCCAAGGA GCAGAGGGAC TCGGACTTGG 421 CGGCCGAGAG TAACTTCCGG ATCTACCCCT GGATGCGAAG CTCAGGAACT GACCGCAAAC 481 GAGGCCGCCA GACCTACACC CGCTACCAGA CCCTGGAGCT GGAGAAAGAA TTTCACTACA 541 ATCGCTACCT GACGCGGCGG CGGCGCATCG AGATCGCGCA CACGCTCTGC CTCACGGAAA 601 GACAGATCAA GATTTGGTTT CAGAACCGGC GCATGAAGTG GAAAAAGGAG AACAAGACCG 661 CGGGCCCGGG GACCACCGGC CAAGACAGGG CTGAAGCAGA GGAGGAAGAG GAAGAGTGCC 721 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 781 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 841 ATATATCTTG TGGAAAGGAC GAAGGGAGCT TCATAGAACT AACTCTACGC GTTAAGTCga 901 caatcaacct ctggattaca aaatttgtga aagatt