Construct: ORF TRCN0000475470
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013252.1_s317c1
- Derived from:
- ccsbBroadEn_03737
- DNA Barcode:
- TCGCTGACGACCGACTCCTCTTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AKIP1 (56672)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475470
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56672 | AKIP1 | A-kinase interacting protein 1 | NM_020642.4 | 100% | 100% | |
| 2 | human | 56672 | AKIP1 | A-kinase interacting protein 1 | XM_017018011.1 | 100% | 100% | |
| 3 | human | 56672 | AKIP1 | A-kinase interacting protein 1 | NM_001206646.2 | 87.1% | 87.1% | 221_222ins81 |
| 4 | human | 56672 | AKIP1 | A-kinase interacting protein 1 | NM_001206647.2 | 87.1% | 87.1% | 408_409ins81 |
| 5 | human | 56672 | AKIP1 | A-kinase interacting protein 1 | NM_001206648.2 | 74.2% | 74.2% | 221_222ins81;327_328ins81 |
| 6 | human | 56672 | AKIP1 | A-kinase interacting protein 1 | XM_017018012.2 | 71% | 63.3% | (many diffs) |
| 7 | human | 56672 | AKIP1 | A-kinase interacting protein 1 | NR_045417.2 | 42.1% | 1_261del;892_1493del | |
| 8 | human | 56672 | AKIP1 | A-kinase interacting protein 1 | NR_045418.2 | 36.7% | 1_261del;482_483ins81;811_1412del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 696
- ORF length:
- 630
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga caactgtttg gcggccgcag cgctgaatgg ggtggaccga cgttccctgc 121 agcgttcagc aaggctggct ctagaagtgc tggagagggc caagaggagg gcggtggact 181 ggcatgccct ggagcgtccc aaaggctgca tgggggtcct tgcccgggag gcgccccacc 241 tagagaaaca gccggcagcc ggcccgcagc gcgttctccc gggagagaga gaagagagac 301 ccccaacccT TAGTGCTTCC TTCAGAACAA TGGCTGAATT CATGGACTAT ACTTCAAGTC 361 AGTGTGGGAA ATATTATTCA TCTGTGCCAG AGGAAGGAGG GGCAACCCAT GTCTATCGTT 421 ATCACAGAGG CGAGTCGAAG CTGCACATGT GCTTGGACAT AGGGAATGGT CAGAGAAAAG 481 ACAGAAAAAA GACATCCCTT GGTCCTGGAG GCAGCTATCA AATATCAGAG CATGCTCCAG 541 AGGCATCCCA GCCTGCTGAG AACATCTCTA AGGACCTCTA CATAGAAGTA TATCCAGGGA 601 CCTATTCTGT CACTGTGGGC TCAAATGACT TAACCAAGAA GACTCATGTG GTAGCAGTTG 661 ATTCTGGACA AAGCGTGGAC CTGGTCTTCC CTGTGTGCCC AACTTTCTTG TACAAAGTGG 721 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 781 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 841 ATCGCTGACG ACCGACTCCT CTTTTACGCG TTAAGTCgac aatcaacctc tggattacaa 901 aatttgtgaa agatt