Construct: ORF TRCN0000475490
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013492.1_s317c1
- Derived from:
- ccsbBroadEn_06127
- DNA Barcode:
- TGTCGATCGAGATACATGGTCGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EPYC (1833)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475490
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1833 | EPYC | epiphycan | NM_004950.5 | 99.8% | 99.6% | 155A>G |
| 2 | human | 1833 | EPYC | epiphycan | XM_011538008.1 | 81% | 81% | 0_1ins183 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1032
- ORF length:
- 966
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gacattagca ggacttgttc tgggacttgt catctttgat gctgctgtga 121 ctgccccaac tctagagtcc atcaactatg actcagaaac ctatgatgcc accttagaag 181 acctggataa tttgtacaac tatgaaaaca tacctgttgg taaagttgag attgaaatag 241 ccacagtgat gccttcaggg aacagagagc tcctcactcc acccccacag cctgagaagg 301 cccaggaaga ggaagaggag gaggaatcta ctcccaggct gattgatggc tcttctcccc 361 aggagcctga attcacaggg gttctggggc cacacacaaa tgaagacttt ccaacctgtc 421 ttttgtgtac ttgtataagt accaccgtgt actgtgatga ccatgaactt gatgctattc 481 ctccgctgcc aaagaacacc gcttatttct attcccgctt taacagaatt aaaaagatca 541 acaaaaatga ctttgcaagc ctaagtgatt taaaaaggat tgatctgaca tcaaatttaa 601 tatctgagat tgatgaagat gcattccGAA AACTGCCTCA ACTTCGAGAG CTTGTCCTGC 661 GTGACAACAA AATAAGGCAG CTCCCAGAAT TGCCAACCAC TTTGACATTT ATTGATATTA 721 GCAACAATAG ACTTGGAAGG AAAGGGATAA AGCAAGAAGC ATTTAAAGAC ATGTATGATC 781 TCCATCATCT GTACCTCACT GATAACAACT TGGACCACAT CCCTCTGCCA CTCCCAGAAA 841 ATCTACGAGC CCTTCACCTC CAGAATAACA ACATTCTGGA AATGCACGAA GATACGTTCT 901 GCAATGTTAA AAATTTGACT TATATTCGTA AGGCACTAGA GGACATTCGA TTGGATGGAA 961 ACCCTATTAA TCTCAGCAAA ACTCCTCAAG CATACATGTG TCTACCTCGT CTGCCTGTTG 1021 GGAGCCTTGT CTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1081 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1141 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATGT CGATCGAGAT ACATGGTCGC 1201 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t