Construct: ORF TRCN0000475519
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008775.1_s317c1
- Derived from:
- ccsbBroadEn_14021
- DNA Barcode:
- CAGTTGGGAACGCAGAATCGGATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EEF1E1 (9521)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475519
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9521 | EEF1E1 | eukaryotic translation elon... | NM_004280.5 | 100% | 100% | |
| 2 | human | 9521 | EEF1E1 | eukaryotic translation elon... | NM_001135650.2 | 78.7% | 74.7% | (many diffs) |
| 3 | human | 100526837 | EEF1E1-BLOC1S5 | EEF1E1-BLOC1S5 readthrough ... | NR_037618.1 | 16.3% | (many diffs) | |
| 4 | mouse | 66143 | Eef1e1 | eukaryotic translation elon... | NM_025380.2 | 86.5% | 88.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 588
- ORF length:
- 522
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcggccgca gagttgtcgc tactggagaa gtccctggga ctgagtaagg 121 ggaataaata cagtgctcag ggcgagcgac agattccagt tcttcagaca aacaatggtc 181 caagtctaac aggattgact actatagcag ctcatctagt caagcaagcC AACAAAGAAT 241 ATTTGCTGGG GAGTACTGCA GAAGAAAAAG CAATCGTTCA GCAGTGGTTA GAATACAGGG 301 TCACTCAAGT AGATGGGCAC TCCAGTAAAA ATGACATCCA CACACTGTTG AAGGATCTTA 361 ATTCATATCT TGAAGATAAA GTCTACCTTA CAGGGTATAA CTTTACATTA GCAGATATAC 421 TATTGTACTA TGGACTTCAT CGCTTTATAG TTGACCTGAC AGTTCAAGAA AAGGAGAAAT 481 ATCTTAATGT GTCTCGCTGG TTTTGTCACA TTCAGCATTA TCCAGGCATC AGGCAACATC 541 TGTCTAGTGT TGTCTTCATC AAGAACAGAC TATATACTAA TTCCCACTAC CCAACTTTCT 601 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 661 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 721 GTGGAAAGGA CGACAGTTGG GAACGCAGAA TCGGATTACG CGTTAAGTCg acaatcaacc 781 tctggattac aaaatttgtg aaagatt