Construct: ORF TRCN0000475528
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002422.1_s317c1
- Derived from:
- ccsbBroadEn_05544
- DNA Barcode:
- CGGAATCGTCTCACAGAGAGCTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RBM43 (375287)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475528
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 375287 | RBM43 | RNA binding motif protein 43 | NM_198557.3 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1140
- ORF length:
- 1071
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcatcagtt ttgaatgtca aggaatccaa agctcctgaa agaacggttg 121 tagttgctgg tcttccagtt gaccttttta gtgatcaatt attggccgta ttagtgaaga 181 gccacttcca agacattaag aatgagggcg gagatgttga agatgtgata tatccgacaa 241 gaaccaaggg agttgcatat gtaatattca aagaaaaaaa agttgcagag aatgtcatca 301 gacaaaagaa acactggcta gcaaggaaga ctagacatgc tgaactcaca gtctctctca 361 gagtctctca ttttggtgac aagatcttca gctctgtaaa tgccatcctt gatctttctg 421 tttttggaaa agaagttact ctagaaactc tggtaaaaga cctgaaaaaa aaaatcccga 481 gtttaagctt cagtcctttg aaacccaatg gaagaatctc cgtggaagga tcatttctgg 541 ctgtcaagag gctcagagaa tctttgctag caagagcatg ttctctctta gaaaaagaca 601 gaaattttac cagtgaggag agaaagtgga atagacaaaa tccccagagg aatctacaga 661 gaagtaataa ctctttggca tcagtcagga ccttagtacc tgagactgct agaagtggag 721 aaatgcttgt gcttgacaca gatgtttttc tttacctgaa acacaagtgt ggatcttatg 781 aaagcacact gaaaaaattc cacattctga gtcaggagaa agtggatggt gaaatcacca 841 caatttgtct aaaaagcatt caagttggtt cTCAGCCAAA CAATGCAAAA CATGTAAAAG 901 AGCTCATTGA GGAATGGTCA CATGCTCTTT ACTTAAAGCT TAGAAAAGAG ACATTTATTT 961 TGGAAGGAAA GGAAAATAGA GAGAAAAGAA TGATCAAAAG GGCATGTGAA CAATTAAGTT 1021 CGAGATACCT TGAAGTCCTG ATTAACCTTT ATAGGACACA CATTGACATT ATAGGATCTT 1081 CTTCTGACAC TTACCTGTTT AAAAAAGGGG TCATGAAATT AATAGGGCAA AAGGTTAGTT 1141 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1201 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1261 TTTATATATC TTGTGGAAAG GACGACGGAA TCGTCTCACA GAGAGCTTGA CGCGTTAAGT 1321 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt