Construct: ORF TRCN0000475537
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003718.1_s317c1
- Derived from:
- ccsbBroadEn_13093
- DNA Barcode:
- TAACTGTATGTGCAGATGAGCTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NAA30 (122830)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475537
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 122830 | NAA30 | N(alpha)-acetyltransferase ... | NM_001011713.3 | 89.7% | 89.7% | 125_235del |
| 2 | mouse | 70646 | Naa30 | N(alpha)-acetyltransferase ... | NM_027492.2 | 79.5% | 79.6% | (many diffs) |
| 3 | mouse | 70646 | Naa30 | N(alpha)-acetyltransferase ... | XM_006519542.3 | 77.2% | 77.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1044
- ORF length:
- 975
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggaggta ccgcctgggc ctagcagcct cctcccacca ccagcacctc 121 cggccccggc ggcggtcgag ccccgctgtc ccttcccggc gggggccgcc ctcgcctgct 181 gcagcgagga cgaggagcag cagcagctca acggattgat tagccccgaa ctgcggcacc 241 tccgggcggc cgcctccctc aagagcaagg tcctgagcgt agcagaggtg gccgcgacca 301 cagccacccc tgacggaggc cccagagcga ctgcaacaaa aggagccggg gtacactcgg 361 gcgagaggcc ccctcactcc ctctctagta atgcaagaac tgcggtcccc agcccggtgg 421 aggcagcggc ggcgagcgat cccgcggcgg cccgcaatgg actggccgag ggcaccgagc 481 aggaggagga ggaggaagac gagcaggtgc ggctgctgtc ttcgtccctg accgccgact 541 gcagcttaag aagcccttcg ggcagggagg ttgagcctgg ggaggatcgg acgatacgat 601 atgtccgata tgaatccgag ctacaaatgc ccgatatcat gagactgatc accaaagatc 661 tgtccgaacc ctactccatt tatacctata gatattttat ccacaactgg ccacagctgt 721 gcttcttggc catggtaggg gaggagtgtg taggtgccat cgtttgcaag ttGGATATGC 781 ACAAAAAGAT GTTCCGCAGA GGTTATATAG CCATGTTAGC CGTGGATTCC AAATACAGGA 841 GAAATGGCAT TGGTACTAAC TTGGTTAAGA AAGCTATATA TGCCATGGTT GAGGGAGACT 901 GTGATGAGGT TGTTTTGGAA ACCGAAATAA CAAATAAGTC CGCTTTGAAA CTTTATGAAA 961 ATCTTGGTTT TGTTCGAGAT AAGAGGCTGT TCAGATACTA TTTAAATGGA GTTGATGCAC 1021 TGCGACTTAA ACTGTGGCTG CGTTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1081 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1141 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT AACTGTATGT 1201 GCAGATGAGC TACACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1261 att