Construct: ORF TRCN0000475560
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018498.1_s317c1
- Derived from:
- ccsbBroadEn_05570
- DNA Barcode:
- AACTCATTCCTTGCGATGAATGGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NHLRC3 (387921)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475560
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 387921 | NHLRC3 | NHL repeat containing 3 | NM_001012754.4 | 100% | 100% | |
| 2 | human | 387921 | NHLRC3 | NHL repeat containing 3 | NM_001017370.2 | 80.6% | 80.6% | 384_385ins201 |
| 3 | human | 387921 | NHLRC3 | NHL repeat containing 3 | NR_073109.1 | 30% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1110
- ORF length:
- 1041
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgagattc tgggtctgcg tagccggtgc tggcttcttt cttgcatttt 121 tggttttgca ttcgcgtttt tgtggctctc cagttttgag gaactttact tttgcagttt 181 cctggagaac tgagaaaatt ctttaccggc tggatgtggg ttggcctaag cacccagaat 241 attttaccgg aacaacattt tgtgttgcag ttgactccct caatggattg gtttacatag 301 gtcaaagagg ggataacatc ccaaagatat tagtgttcac agaggatgga tatttcctac 361 gagcctggaa ttatacagtt gacacacctc atggtatatt tgcagccagt actctatatg 421 aacaatccgt ctggatcacg gatgtaggaa gtggattctt tggtcatact gttaaaaaat 481 acagttcttt tggtgatctt gttcaagtct tgggtactcc aggcaaaaaa ggcactagtt 541 tgaatccttt gcagtttgat aacccagcag aattatatgt agaggacaca ggagatattt 601 acattgtgga tggagatgga ggattgaata acagattgat caaactgtcc caagatttca 661 tgatcctttg gctgcatgga gaaaatggga cagggcctgc taagttcaac atacctcaca 721 gtgttacact tgattcagct ggtcgggtgt gggttgctga ccgaggaaat aaaagaatcc 781 aagtatttga taaagacact ggggagtggt taggagcatg gaataattgt ttcacagaag 841 agggaccttc ttcagtcagg tttactcctg atgggaagta cttgattgtg gcccagctga 901 atcttagcag gctcTCAGTC GTAGCAGCAC CCCCAGTGGG AAGCATTGGG GAGTGTTCTG 961 TGATCAGCAC AATCCAACTA GCAGATCAAG TTTTGCCACA TCTCCTAGAA GTCGACAGAA 1021 AGACTGGAGC AGTCTATGTA GCAGAAATTG GAGCAAAACA AGTACAAAAA TATGTCCCTT 1081 TGAATAGCTA TGTTCCTTCA TTTGGTTCAT TGCCAACTTT CTTGTACAAA GTGGTTGATA 1141 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1201 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAAACTC 1261 ATTCCTTGCG ATGAATGGCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1321 tgaaagatt