Construct: ORF TRCN0000475585
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000189.1_s317c1
- Derived from:
- ccsbBroadEn_00212
- DNA Barcode:
- CCGCATAAGGCCAGATCGGTGCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CAMLG (819)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475585
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 819 | CAMLG | calcium modulating ligand | NM_001745.4 | 100% | 100% | |
| 2 | mouse | 12328 | Caml | calcium modulating ligand | NM_007596.2 | 83.1% | 88.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 957
- ORF length:
- 888
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagtcgatg gccgtcgcta ccgacggcgg ggagaggccg ggggtcccag 121 cgggctcagg tctgtcggct tcccagcgtc gggcggagct gcgtcggaga aagctgctca 181 tgaactcgga acagcgcatc aaccggatca tgggctttca caggcccggg agcggcgcgg 241 aagaagaaag tcaaacaaaa tcaaagcagc aggacagtga taaactgaac tccctcagcg 301 ttccttccgt ttcaaagcga gtagtgctgg gtgattcagt cagtacagga acaactgacc 361 agcagggtgg tgtggccgag gtaaagggga cccaactggg agacaaattg gactcgttca 421 ttaaaccacc tgagtgcagt agtgatgtca accttgagct ccggcagcgg aacagagggg 481 acctgacagc ggactcggtc cagaggggtt cccgccatgg cctagagcag tacctttcca 541 gattcgaaga agcaatgaag ctaaggaaac agctgattag tgaaaaaccc agtcaagagg 601 atggaaatac aacagaagaa tttgactctt ttcgaatatt tagattggtg ggatgtgctc 661 ttcttgctct tGGAGTCAGA GCTTTTGTTT GCAAATACTT GTCCATATTT GCTCCATTTC 721 TTACTTTACA ACTTGCGTAC ATGGGATTAT ACAAATATTT TCCCAAGAGT GAAAAGAAGA 781 TAAAGACAAC AGTACTAACA GCTGCACTTC TATTGTCGGG AATTCCTGCC GAAGTGATAA 841 ATCGATCAAT GGATACCTAT AGCAAAATGG GCGAAGTCTT CACAGATCTC TGTGTCTACT 901 TTTTCACTTT TATCTTTTGT CATGAACTGC TTGATTATTG GGGCTCTGAA GTACCATTGC 961 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1021 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1081 ATATATCTTG TGGAAAGGAC GACCGCATAA GGCCAGATCG GTGCTCACGC GTTAAGTCga 1141 caatcaacct ctggattaca aaatttgtga aagatt