Construct: ORF TRCN0000475588
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013041.1_s317c1
- Derived from:
- ccsbBroadEn_01589
- DNA Barcode:
- ATCGGATTTGTCAAAACTGAAAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SQLE (6713)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475588
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6713 | SQLE | squalene epoxidase | NM_003129.4 | 100% | 100% | |
| 2 | human | 6713 | SQLE | squalene epoxidase | XM_011517246.2 | 87.5% | 84.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1788
- ORF length:
- 1722
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg gacttttctg ggcattgcca ctttcaccta tttttataag aagttcgggg 121 acttcatcac tttggccaac agggaggtcc tgttgtgcgt gctggtgttc ctctcgctgg 181 gcctggtgct ctcctaccgc tgtcgccacc gaaacggggg tctcctcggg cgccagcaga 241 gcggctccca gttcgccctc ttctcggata ttctctcagg cctgcctttc attggcttct 301 tctgggccaa atccccccct gaatcagaaa ataaggagca gctcgaggcc aggaggcgca 361 gaaaaggaac caatatttca gaaacaagct taataggaac agctgcctgt acatcaacat 421 cttctcagaa tgacccagaa gttatcatcg tgggagctgg cgtgcttggc tctgctttgg 481 cagctgtgct ttccagagat ggaagaaagg tgacagtcat tgagagagac ttaaaagagc 541 ctgacagaat agttggagaa ttcctgcagc cgggtggtta tcatgttctc aaagaccttg 601 gtcttggaga tacagtggaa ggtcttgatg cccaggttgt aaatggttac atgattcatg 661 atcaggaaag caaatcagag gttcagattc cttaccctct gtcagaaaac aatcaagtgc 721 agagtggaag agctttccat cacggaagat tcatcatgag tctccggaaa gcagctatgg 781 cagagcccaa tgcaaagttt attgaaggtg ttgtgttaca gttattagag gaagatgatg 841 ttgtgatggg agttcagtac aaggataaag agactggaga tatcaaggaa ctccatgctc 901 cactgactgt tgttgcagat gggcttttct ccaagttcag gaaaagcctg gtctccaata 961 aagtttctgt atcatctcat tttgttggct ttcttatgaa gaatgcacca cagtttaaag 1021 caaatcatgc tgaacttatt ttagctaacc cgagtccagt tctcatctac cagatttcat 1081 ccagtgaaac tcgagtactt gttgacatta gaggagaaat gccaaggaat ttaagagaat 1141 acatggttga aaaaatttac ccacaaatac ctgatcacct gaaagaacca ttcttagaag 1201 ccactgacaa ttctcatctg aggtccatgc cagcaagctt ccttcctcct tcatcagtga 1261 agaaacgagg tgttcttctt ttgggagacg catataatat gaggcatcca cttactggtg 1321 gaggaatgac tgttgctttt aaagatataa aactatggag aaaactgcta aagggtatcc 1381 ctgaccttta tgatgatgca gctattttcg aggccaaaaa atcattttac tgggcaagaa 1441 aaacatctca ttcctttgtc gtgaatatcc ttgctcaggc tctttatgaa ttatttTCTG 1501 CCACAGATGA TTCCCTGCAT CAACTAAGAA AAGCCTGTTT TCTTTATTTC AAACTTGGTG 1561 GCGAATGTGT TGCGGGTCCT GTTGGGCTGC TTTCTGTATT GTCTCCTAAC CCTCTAGTTT 1621 TAATTGGACA CTTCTTTGCT GTTGCAATCT ATGCCGTGTA TTTTTGCTTT AAGTCAGAAC 1681 CTTGGATTAC AAAACCTCGA GCCCTTCTCA GTAGTGGTGC TGTATTGTAC AAAGCGTGTT 1741 CTGTAATATT TCCTCTAATT TACTCAGAAA TGAAGTATAT GGTTCATTAC CCAACTTTCT 1801 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1861 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1921 GTGGAAAGGA CGAATCGGAT TTGTCAAAAC TGAAAGCACG CGTTAAGTCg acaatcaacc 1981 tctggattac aaaatttgtg aaagatt