Construct: ORF TRCN0000475590
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015772.1_s317c1
- Derived from:
- ccsbBroadEn_10163
- DNA Barcode:
- GGGAAGTAATAAACTTTTGATTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PLIN5 (440503)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475590
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 440503 | PLIN5 | perilipin 5 | NM_001013706.3 | 99.9% | 99.7% | 763T>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1458
- ORF length:
- 1389
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtctgaagaa gaggcggctc agatccccag atccagtgtg tgggagcagg 121 accagcagaa cgtggtgcag cgtgtggtgg ctctgcccct ggtcagggcc acgtgcaccg 181 cggtctgcga tgtttacagt gcagccaagg acaggcaccc gctgctgggc tccgcctgcc 241 gcctggctga gaactgcgtg tgcggcctga ccacccgtgc cctggaccac gcccagccgc 301 tgctcgagca cctgcagccc cagctggcca ctatgaacag cctcgcctgc aggggcctgg 361 acaagctgga agagaagctt ccctttctcc agcaaccttc ggagacggtg gtgacctcag 421 ccaaggacgt ggtggccagc agtgtcacgg gtgtggtgga cctggcccgg aggggccggc 481 gctggagcgt ggagctgaag cgctccgtga gccatgctgt ggatgttgta ctggaaaaat 541 cagaggagct ggtggatcac ttcctgccca tgacggagga agagctcgcg gcactggcgg 601 ctgaggctga aggccctgaa gtgggttcgg tggaggatca gaggagacag cagggctact 661 ttgtgcgcct cggctccctg tcagcacgga tccgccacct ggcctacgag cactctgtgg 721 ggaaactgag gcagagcaaa caccgtgccc aggacaccct ggcccagctg caggagacgc 781 tggagctgat agaccacatg cagtgtgggg tgacccccac cgccccggcc cgccctggga 841 aggtgcacga gctgtggggg gaatggggcc agcgccctcc ggagagccgc cgccggagcc 901 aggcagagct ggagacgctg gtgctgtccc gcagcctgac ccaggagctg cagggcacgg 961 tagaggctct ggagtccagc gtgcggggcc tgcccgccgg cgcccaggag aaggtggctg 1021 aggtgcggcg cagtgtggat gccctgcaga ccgccttcgc tgatgcccgc tgcttcaggg 1081 acgtgccagc ggccgcgctg gccgagggcc ggggtcgcgt ggcccacgcg cacgccTGCG 1141 TGGACGAGCT GCTGGAGCTG GTGGTGCAGG CCGTGCCGCT GCCCTGGCTG GTGGGACCCT 1201 TCGCGCCCAT CCTTGTGGAG CGACCCGAGC CCCTGCCCGA CCTGGCGGAC CTGGTGGACG 1261 AGGTCATCGG GGGCCCTGAC CCCCGCTGGG CGCACCTGGA CTGGCCGGCC CAGCAGAGAG 1321 CCTGGGAGGC AGAGCACAGG GACGGGAGTG GGAATGGGGA TGGGGACAGG ATGGGTGTTG 1381 CCGGGGACAT CTGCGAGCAG GAACCCGAGA CCCCCAGCTG CCCGGTCAAG CACACCCTGA 1441 TGCCCGAGCT GGACTTCTTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1501 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1561 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGGGAAGT AATAAACTTT 1621 TGATTTAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt