Construct: ORF TRCN0000475593

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015370.1_s317c1
Derived from:
ccsbBroadEn_02772
DNA Barcode:
TGCTAAATATTCTGCCCGACCCTC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ICMT (23463)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000475593
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23463 ICMT isoprenylcysteine carboxyl ... NM_012405.4 100% 100%
2 human 23463 ICMT isoprenylcysteine carboxyl ... XM_011541140.2 66.1% 66.1% 0_1ins288
3 mouse 57295 Icmt isoprenylcysteine carboxyl ... NM_133788.2 89% 96.1% (many diffs)
4 mouse 57295 Icmt isoprenylcysteine carboxyl ... NR_037908.1 14.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
918
ORF length:
852
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc gggctgcgcg gcgcgggctc cgccgggctc tgaggcgcgt ctcagcctcg
121ccaccttcct gctgggcgcc tcggtgctcg cgctgccgct gctcacgcgc gccggcctgc
181agggccgcac cgggctggcg ctctacgtgg ccgggctcaa cgcgctgctg ctgctgctct
241atcggccgcc tcgctaccag atagccatcc gagcttgttt cctggggttt gtgttcggct
301gcggcacgct gctaagtttt agccagtctt cttggagtca ctttggctgg tacatgtgct
361ccctgtcatt gttccactat tctgaatact tggtgacagc agtcaataat cccaaaagtc
421tgtccttgga ttcctttctc ctgaatcaca gcctggagta tacagtagct gctctttctt
481cttggttaga gttcacactt gaaaatatct tttggccaga actgaagcag attacctggc
541tcagtgtcac agggctgctg atggtggtct tcggagaatg tctgaggaag gcggccatgt
601ttacagctgg ctccaatttc aaccacgtgg tacagaatga aaaaTCAGAT ACACATACTC
661TGGTGACCAG TGGAGTGTAC GCTTGGTTTC GGCATCCTTC TTACGTCGGG TGGTTTTACT
721GGAGTATTGG AACTCAGGTG ATGCTGTGTA ACCCCATCTG CGGCGTCAGC TATGCCCTGA
781CAGTGTGGCG ATTCTTCCGC GATCGAACAG AAGAAGAAGA AATCTCACTA ATTCACTTTT
841TTGGAGAGGA GTACCTGGAG TATAAGAAGA GGGTGCCCAC GGGCCTGCCT TTCATAAAGG
901GGGTCAAGGT GGACCTGTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA
961TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA
1021GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATGCTAAA TATTCTGCCC
1081GACCCTCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)