Construct: ORF TRCN0000475601
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009662.1_s317c1
- Derived from:
- ccsbBroadEn_00788
- DNA Barcode:
- GATTTCCGGTGCCTGGTGGAGGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HRH2 (3274)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475601
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3274 | HRH2 | histamine receptor H2 | NM_001131055.2 | 100% | 100% | |
| 2 | human | 3274 | HRH2 | histamine receptor H2 | XM_006714865.3 | 91% | 90.4% | 1075_1076ins34;1082_1082delCinsGACCCA;1086_1087ins66 |
| 3 | human | 3274 | HRH2 | histamine receptor H2 | NM_001367711.1 | 86.9% | 82.9% | (many diffs) |
| 4 | human | 3274 | HRH2 | histamine receptor H2 | XM_006714864.4 | 86.9% | 82.9% | (many diffs) |
| 5 | human | 3274 | HRH2 | histamine receptor H2 | XM_011534548.3 | 86.9% | 82.9% | (many diffs) |
| 6 | human | 3274 | HRH2 | histamine receptor H2 | XM_011534549.3 | 86.9% | 82.9% | (many diffs) |
| 7 | human | 3274 | HRH2 | histamine receptor H2 | XM_017009426.2 | 86.9% | 82.9% | (many diffs) |
| 8 | human | 3274 | HRH2 | histamine receptor H2 | NR_160284.1 | 23.8% | (many diffs) | |
| 9 | mouse | 15466 | Hrh2 | histamine receptor H2 | XM_006516853.3 | 76.3% | 75.5% | (many diffs) |
| 10 | mouse | 15466 | Hrh2 | histamine receptor H2 | NM_008286.2 | 75.8% | 77.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1260
- ORF length:
- 1191
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcacccaat ggcacagcct cttccttttg cctggactct accgcatgca 121 agatcaccat caccgtggtc cttgcggtcc tcatcctcat caccgttgct ggcaatgtgg 181 tcgtctgtct ggccgtgggc ttgaaccgcc ggctccgcaa cctgaccaat tgtttcatcg 241 tgtccttggc tatcactgac ctgctcctcg gcctcctggt gctgcccttc tctgccatct 301 accagctgtc ctgcaagtgg agctttggca aggtcttctg caatatctac accagcctgg 361 atgtgatgct ctgcacagcc tccattctta acctcttcat gatcagcctc gaccggtact 421 gcgctgtcat ggacccactg cggtaccctg tgctggtcac cccagttcgg gtcgccatct 481 ctctggtctt aatttgggtc atctccatta ccctgtcctt tctgtctatc cacctggggt 541 ggaacagcag gaacgagacc agcaagggca atcataccac ctctaagtgc aaagtccagg 601 tcaatgaagt gtacgggctg gtggatgggc tggtcacctt ctacctcccg ctactgatca 661 tgtgcatcac ctactaccgc atcttcaagg tcgcccggga tcaggccaag aggatcaatc 721 acattagctc ctggaaggca gccaccatca gggagcacaa agccacagtg acactggccg 781 ccgtcatggg ggccttcatc atctgctggt ttccctactt caccgcgttt gtgtaccgtg 841 ggctgagagg ggatgatgcc atcaatgagg tgttagaagc catcgttctg tggctgggct 901 atgccaactc agccctgaac cccatcctgt atgctgcgct gaacagagac ttccgcaccg 961 ggtaccaaca gctcttctgc tgcaggctgg ccaaccgcaa ctcccacaaa acttctctga 1021 ggtccaacgc ctctcagctg tccaggaccc aaagccgaga acccaggcaa caggaagaga 1081 aacccctgaa gctccaggtg tggagtggga cagaagtCAC GGCCCCCCAG GGAGCCACAG 1141 ACAGACCATG GCTTTGCCTT CCAGAATGCT GGTCTGTGGA ACTGACCCAT TCATTCATTC 1201 ATTTGTTCAT TCATTCATTT GCAAACATTC ATCCAATTCC CACCACATGC CAGGAATTAT 1261 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1321 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1381 TTTATATATC TTGTGGAAAG GACGAGATTT CCGGTGCCTG GTGGAGGCAA CGCGTTAAGT 1441 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt