Construct: ORF TRCN0000475620
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005997.1_s317c1
- Derived from:
- ccsbBroadEn_05566
- DNA Barcode:
- GACCTCCCGTGGCTGATATGTTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TEX36 (387718)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475620
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 387718 | TEX36 | testis expressed 36 | NM_001128202.3 | 100% | 100% | |
| 2 | human | 387718 | TEX36 | testis expressed 36 | NM_001318133.2 | 58.5% | 46.3% | (many diffs) |
| 3 | human | 387718 | TEX36 | testis expressed 36 | XM_005269817.4 | 51.3% | 47.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 627
- ORF length:
- 558
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaccaaaggg cgacgcttca acccaccttc agacaaggat gggagatggt 121 tccctcacat cgggctaacg caaaagacac cagaatccat caccagtgct acgtcaaaag 181 agccccagag tccacacttg cctcggcaag cggaggggaa gctgccgccc atatacaaag 241 tccgggagaa gcaagcagtg aataaccagt tccccttctc cgtgcatgac aatcggcaca 301 gcttggagaa ctctggatgc taccttgact ccggccTGGG ACGTAAGAAG ATCTCTCCAG 361 ATAAGAGGCA ACATGTTTCA AGAAATTTTA ATCTCTGGGC ATGTGACTAT GTTCCATCTT 421 GTCTTGATGG CTTTTCAAAT AACCAAATAT CATACGTATA TAAAGAAGCC ATGGTGGTCT 481 CAAGCTTCAG ACGCTTTCCA CGATGCTATA AAGAGATATG GAACGCTTTT ACATTTCTTC 541 CTGAGAGAAG CTATACAGAG GTTTTGAAAA AGAAGCCCAA AGTAAGGTTC ACTGTTGACA 601 AAAAGGTTGT TTCTTCACTG GAGTCCTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 661 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 721 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGACCTCCC 781 GTGGCTGATA TGTTTGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 841 aagatt