Construct: ORF TRCN0000475634
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013081.1_s317c1
- Derived from:
- ccsbBroadEn_08040
- DNA Barcode:
- GGGAATGAGATGCCTTCGGCGATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FGF22 (27006)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475634
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27006 | FGF22 | fibroblast growth factor 22 | NM_020637.1 | 100% | 100% | |
| 2 | human | 27006 | FGF22 | fibroblast growth factor 22 | NM_001300812.2 | 94% | 64.3% | 317_318ins23;488_495delTGAGGCCC |
| 3 | mouse | 67112 | Fgf22 | fibroblast growth factor 22 | XM_006513973.2 | 63.9% | 49.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 579
- ORF length:
- 510
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcgccgccgc ctgtggctgg gcctggcctg gctgctgctg gcgcgggcgc 121 cggacgccgc gggaaccccg agcgcgtcgc ggggaccgcg cagctacccg cacctggagg 181 gcgacgtgcg ctggcggcgc ctcttctcct ccactcactt cttcctgcgc gtggatcccg 241 gcggccgcgt gcagggcacc cgctggcgcc acggccagga cagcatcctg gagatccgct 301 ctgtacacgt gggcgtcgtg gtcatcaaag cagtgtcctc aggcttctac gtggccatga 361 accgccgggg ccgcctctac gggtcgcgac tctacaccgt ggactgcagg ttccgggagc 421 gcatcgaaga gaacggccac aacaccTACG CCTCACAGCG CTGGCGCCGC CGCGGCCAGC 481 CCATGTTCCT GGCGCTGGAC AGGAGGGGGG GGCCCCGGCC AGGCGGCCGG ACGCGGCGGT 541 ACCACCTGTC CGCCCACTTC CTGCCCGTCC TGGTCTCCTT GCCAACTTTC TTGTACAAAG 601 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 661 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 721 ACGAGGGAAT GAGATGCCTT CGGCGATCAC GCGTTAAGTC gacaatcaac ctctggatta 781 caaaatttgt gaaagatt