Construct: ORF TRCN0000475638
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006611.1_s317c1
- Derived from:
- ccsbBroadEn_12235
- DNA Barcode:
- ACCGTCTACTATACCACAAGTACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRR5 (55615)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475638
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55615 | PRR5 | proline rich 5 | NM_001017529.2 | 97% | 95.9% | (many diffs) |
| 2 | human | 55615 | PRR5 | proline rich 5 | NM_001017530.1 | 97% | 95.9% | (many diffs) |
| 3 | human | 55615 | PRR5 | proline rich 5 | NM_001017528.2 | 75% | 74.1% | (many diffs) |
| 4 | human | 55615 | PRR5 | proline rich 5 | NM_015366.4 | 75% | 74.1% | (many diffs) |
| 5 | human | 55615 | PRR5 | proline rich 5 | NM_181333.4 | 73.2% | 72.4% | (many diffs) |
| 6 | human | 55615 | PRR5 | proline rich 5 | NM_001198721.2 | 69.1% | 68.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 927
- ORF length:
- 861
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ccccatgccc acaggacaga agctgctgga ctcactggca gagacctggg 121 acttcttctt cagtgacgtg ctgcccatgc tgcaggccat cttctacccg gtgcagggca 181 aggagccatc ggtgcgccag ctggccctgc tgcacttccg gaatgccatc accctcagtg 241 tgaagctaga ggatgcgctg gcccgggccc atgcccgcgt gccccctgcc atcgtgcaga 301 tgctgctggt gctgcagggg gtacatgagt ccaggggcgt gactgaggac tacctgcgcc 361 tggagacgct ggtccagaag gtggtgtcgc catacctggg cacctacggc ctccactcca 421 gcgaggggcc cttcacccat tcctgcatcc tggaaaagcg cctcctccgc cgctcccgct 481 cgggggacgt gctggccaag aaccctgtgg tgcgctccaa gagctacaac acgcctctgc 541 tgaaccccgt gcaggagcac gaggcggagg gcgcggcggc cggcggtacc agcatccgca 601 ggcactctgt gtcggagatg acgtcctgcc ccgagcctca gggcttcTCC GACCCGCCCG 661 GCCAGGGCCC CACCGGGACC TTCAGGTCCT CCCCGGCGCC CCACTCAGGG CCCTGCCCCA 721 GCAGACTGTA CCCCACGACC CAGCCCCCTG AGCAGGGCTT GGATCCCACC CGCAGCTCCC 781 TGCCCCGCTC CAGCCCGGAG AACCTGGTGG ACCAGATCCT GGAGTCCGTG GACTCGGATT 841 CTGAAGGGAT TTTCATTGAC TTTGGCCGGG GCCGGGGCTC TGGCATGTCC GACTTGGAGG 901 GCTCTGGGGG CCGGCAGAGT GTCGTGTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 961 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1021 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAACCGTCTA 1081 CTATACCACA AGTACCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1141 aagatt