Construct: ORF TRCN0000475643
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004369.1_s317c1
- Derived from:
- ccsbBroadEn_09611
- DNA Barcode:
- GCAATCGCATACGGTTGTGTTTGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- E2F7 (144455)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475643
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 144455 | E2F7 | E2F transcription factor 7 | NM_203394.3 | 99.8% | 99.7% | 214T>C;969A>G;2721C>A |
| 2 | human | 144455 | E2F7 | E2F transcription factor 7 | XM_011537966.2 | 94.9% | 94.7% | (many diffs) |
| 3 | human | 144455 | E2F7 | E2F transcription factor 7 | XM_011537969.2 | 85.9% | 86.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2802
- ORF length:
- 2733
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaggtaaat tgtttaacac taaaagacct gatcagcccc aggcagccca 121 gactagattt tgcagttgaa gatggggaaa atgcacaaaa ggaaaatata tttgttgatc 181 gatcaaggat ggccccgaag actccaataa aaaatgaacc aattgattta tcgaagcaaa 241 aaaaatttac tccagaaaga aatcccatta ctccagttaa gcttgttgac agacagcaag 301 cggaaccatg gacacccaca gctaacctga agatgctcat tagtgctgcc agcccagata 361 taagggaccg ggagaagaaa aagggactat tccgacccat tgaaaacaag gacgatgcat 421 ttacagattc tctacagctt gatgttgttg gggacagtgc tgtggacgaa tttgaaaagc 481 aaaggccaag cagaaaacag aaaagtttag gactcctgtg ccagaagttt ctagctcgct 541 atccaagtta tcccttgtca actgagaaaa ctaccatctc cctagatgaa gttgctgtca 601 gtcttggtgt ggaaaggaga cgcatctatg acattgtaaa tgtgctggag tcgctgcatc 661 tggtcagccg ggtggctaag aatcagtatg gctggcatgg acggcacagc ctgccaaaaa 721 ccctgaggaa cctccagaga ctaggagagg agcagaaata tgaagagcaa atggcctacc 781 tccaacagaa agagctggac ctgatagatt ataaatttgg agaacgtaaa aaagatggtg 841 atccagattc ccaggaacaa cagttactgg atttctctga acccgactgt ccctcttcat 901 ctgcaaacag tagaaaagac aagtctctga gaattatgag ccagaagttt gtcatgctgt 961 tcctcgtctc caaaaccaag attgtcactc tggatgtggc tgccaaaata ctgatagaag 1021 aaagccaaga tgccccggac catagtaaat ttaaaacaaa ggtacgacgc ctctatgaca 1081 tagccaatgt tctgaccagc ttggctctga taaagaaagt gcatgtaaca gaagagcgag 1141 gtcgtaaacc agccttcaag tggatcgggc ctgtggactt cagctcaagt gatgaagaac 1201 tggtggatgt ttctgcatct gtcttaccag aattgaaaag agaaacatat ggccagattc 1261 aagtctgtgc aaaacagaag ctggctcgcc atggttcttt taacacagtt caggcttctg 1321 agaggatcca gaggaaagtg aactcagaac cgagcagccc gtacagagaa gaacaaggat 1381 caggtggcta ctctttagaa attggaagcc tggcagctgt ctatagacag aaaatagaag 1441 acaattcaca gggaaaagcc tttgccagta agagagtggt gcctccatca agcagcttgg 1501 accctgttgc tcctttccct gtcctctctg ttgacccaga atattgtgtt aatcctttag 1561 cccacccagt attttctgtt gctcagacgg acctgcaggc attctccatg cagaacggtc 1621 tgaatggaca agtggatgtc tcacttgctt ctgcagcctc tgctgtggag agcctgaagc 1681 cagcactcct tgctggccag cctctagtgt atgtgccctc tgcctcactg ttcatgctgt 1741 atggaagtct gcaggaggga ccagcgtcag ggtcagggtc agagagggat gacagaagct 1801 cagaagcccc agccacagta gagctgtcat ctgcaccctc agctcagaag cgcctctgtg 1861 aggagaggaa acctcaggag gaggatgagc cagccactaa aaggcaaagt agggaatatg 1921 aagacggccc gctgtcgctt gtcatgccca agaaaccctc agattccaca gaccttgcct 1981 ctcccaagac tatgggtaac agggcatcta tacccctcaa agacattcat gtgaatggcc 2041 aactccctgc tgcagaagag atttcaggaa aggcaacagc aaactctctt gtttcttctg 2101 agtggggaaa tccttcaaga aatacagatg ttgaaaagcc ttcaaaagaa aatgaaagca 2161 ccaaagagcc ttctttgcta caatatcttt gtgtgcagtc tcctgcagga ttaaatggtt 2221 tcaatgtact tttatctggc agtcaaaccc cccctactgt gggcccgtcc tcaggtcagc 2281 tgccgtcttt cagtgtccct tgcatggtct taccatctcc acctctgggc ccttttcctg 2341 ttctctattc tcctgcaatg ccgggcccgg tttcttctac tcttggtgct ctcccaaaca 2401 caggacctgt gaatttcagc ttgcctggcc ttggatcaat agcccagctt ctcgtcggcc 2461 ccacagctgt ggttaatcca aagtcgtcca cactcccttc tgcagacccT CAGCTTCAGA 2521 GTCAGCCCTC ACTAAACCTA AGTCCAGTGA TGTCAAGGTC ACACAGTGTC GTCCAACAAC 2581 CTGAGTCCCC CGTTTACGTG GGACATCCAG TCTCAGTAGT AAAATTACAT CAGTCACCAG 2641 TTCCAGTGAC CCCCAAGAGC ATCCAACGCA CACATCGTGA GACGTTTTTC AAGACACCCG 2701 GCAGCCTTGG AGACCCTGTC CTGAAGAGAA GAGAAAGGAA CCAGTCACGA AACACCAGCT 2761 CGGCCCAGAG GAGACTAGAA ATCCCCAGAG GCGGCGCTGA CTTGCCAACT TTCTTGTACA 2821 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 2881 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 2941 AGGACGAGCA ATCGCATACG GTTGTGTTTG AACGCGTTAA GTCgacaatc aacctctgga 3001 ttacaaaatt tgtgaaagat t