Construct: ORF TRCN0000475652
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009720.1_s317c1
- Derived from:
- ccsbBroadEn_06193
- DNA Barcode:
- CTGACTCCCATGGCAATGATCAGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ACSL3 (2181)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475652
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2181 | ACSL3 | acyl-CoA synthetase long ch... | NM_001354158.2 | 99.9% | 99.8% | 11A>T;1620C>T |
| 2 | human | 2181 | ACSL3 | acyl-CoA synthetase long ch... | NM_001354159.2 | 99.9% | 99.8% | 11A>T;1620C>T |
| 3 | human | 2181 | ACSL3 | acyl-CoA synthetase long ch... | NM_004457.5 | 99.9% | 99.8% | 11A>T;1620C>T |
| 4 | human | 2181 | ACSL3 | acyl-CoA synthetase long ch... | NM_203372.3 | 99.9% | 99.8% | 11A>T;1620C>T |
| 5 | mouse | 74205 | Acsl3 | acyl-CoA synthetase long-ch... | NM_001033606.2 | 88.8% | 92.7% | (many diffs) |
| 6 | mouse | 74205 | Acsl3 | acyl-CoA synthetase long-ch... | NM_028817.3 | 88.8% | 92.7% | (many diffs) |
| 7 | mouse | 74205 | Acsl3 | acyl-CoA synthetase long-ch... | NM_001136222.1 | 70.4% | 74.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2229
- ORF length:
- 2160
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcagcat gaataacctc gtgtcttcaa aaccatctac catgaagcta aaacatacca 121 tcaaccctat tcttttatat tttatacatt ttctaatatc actttatact attttaacat 181 acattccgtt ttattttttc tccgagtcaa gacaagaaaa atcaaaccga attaaagcaa 241 agcctgtaaa ttcaaaacct gattctgcat acagatctgt taatagtttg gatggtttgg 301 cttcagtatt ataccctgga tgtgatactt tagataaagt ttttacatat gcaaaaaaca 361 aatttaagaa caaaagactc ttgggaacac gtgaagtttt aaatgaggaa gatgaagtac 421 aaccaaatgg aaaaattttt aaaaaggtta ttcttggaca gtataattgg ctttcctatg 481 aagatgtctt tgttcgagcc tttaattttg gaaatggatt acagatgttg ggtcagaaac 541 caaagaccaa catcgccatc ttctgtgaga ccagggccga gtggatgata gctgcacagg 601 cgtgttttat gtataatttt cagcttgtta cattatatgc cactctagga ggtccagcca 661 ttgttcatgc attaaatgaa acagaggtga ccaacatcat tactagtaaa gaactcttac 721 aaacaaagtt gaaggatata gtttctttgg tcccacgcct gcggcacatc atcactgttg 781 atggaaagcc accgacctgg tccgagttcc ccaagggcat cattgtgcat accatggctg 841 cagtggaggc cctgggagcc aaggccagca tggaaaacca acctcatagc aaaccattgc 901 cctcagatat tgcagtaatc atgtacacaa gtggatccac aggacttcca aagggagtca 961 tgatctcaca tagtaacatt attgctggta taactgggat ggcagaaagg attccagaac 1021 taggagagga agatgtctac attggatatt tgcctctggc ccatgttcta gaattaagtg 1081 ctgagcttgt ctgtctttct cacggatgcc gcattggtta ctcttcacca cagactttag 1141 cagatcagtc ttcaaaaatt aaaaaaggaa gcaaagggga tacatccatg ttgaaaccaa 1201 cactgatggc agcagttccg gaaatcatgg atcggatcta caaaaatgtc atgaataaag 1261 tcagtgaaat gagtagtttt caacgtaatc tgtttattct ggcctataat tacaaaatgg 1321 aacagatttc aaaaggacgt aatactccac tgtgcgacag ctttgttttc cggaaagttc 1381 gaagcttgct agggggaaat attcgtctcc tgttgtgtgg tggcgctcca ctttctgcaa 1441 ccacgcagcg attcatgaac atctgtttct gctgtcctgt tggtcaggga tacgggctca 1501 ctgaatctgc tggggctgga acaatttccg aagtgtggga ctacaatact ggcagagtgg 1561 gagcaccatt agtttgctgt gaaatcaaat taaaaaactg ggaggaaggt ggatacttta 1621 atactgataa gccacacccc aggggtgaaa ttcttattgg gggccaaagt gtgacaatgg 1681 ggtactataa aaatgaagca aaaacaaaag ctgatttctt tgaagatgaa aatggacaaa 1741 ggtggctctg tactggggat attggagagt ttgaacccga tggatgctta aagattattg 1801 atcgtaaaaa ggaccttgta aaactacagg caggggaata tgtttctctt gggaaagtag 1861 aggcagcttt gaagaatctt ccactagtag ataacatttg tgcatatgca aacagttatc 1921 attcttatgt cattGGATTT GTTGTGCCAA ATCAAAAGGA ACTAACTGAA CTAGCTCGAA 1981 AGAAAGGACT TAAAGGGACT TGGGAGGAGC TGTGTAACAG TTGTGAAATG GAAAATGAGG 2041 TACTTAAAGT GCTTTCCGAA GCTGCTATTT CAGCAAGTCT GGAAAAGTTT GAAATTCCAG 2101 TAAAAATTCG TTTGAGTCCT GAACCGTGGA CCCCTGAAAC TGGTCTGGTG ACAGATGCCT 2161 TCAAGCTGAA ACGCAAAGAG CTTAAAACAC ATTACCAGGC GGACATTGAG CGAATGTATG 2221 GAAGAAAATT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 2281 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 2341 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACTGACT CCCATGGCAA TGATCAGTAC 2401 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt