Construct: ORF TRCN0000475666
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001744.1_s317c1
- Derived from:
- ccsbBroadEn_10923
- DNA Barcode:
- CTTAACGTGAATCTACTACTTAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- INSL3 (3640)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475666
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3640 | INSL3 | insulin like 3 | NM_005543.4 | 99.4% | 99.2% | 126A>G;178A>G |
| 2 | human | 3640 | INSL3 | insulin like 3 | NM_001265587.2 | 76.6% | 39.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 462
- ORF length:
- 393
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggacccccgt ctgcccgcct gggcgctggt gctgctgggc cctgccctgg 121 tgttcgcgtt gggccccgcg cccaccccag agatgcgtga gaagttgtgc ggccaccact 181 tcgtacgcgc gctggtgcgc gtgtgcgggg gcccccgctg gtccaccgaa gccaggaggc 241 cTGCGGCCGG AGGCGACCGT GAGTTGCTAC AGTGGCTGGA GAGACGACAT CTGCTCCATG 301 GGCTGGTGGC CGACAGTAAT CTCACGCTGG GACCTGGCCT GCAGCCCCTG CCCCAGACCT 361 CTCACCATCA CCGCCACCAC CGTGCAGCTG CCACCAACCC TGCACGCTAC TGCTGCCTCA 421 GTGGCTGTAC CCAACAAGAC CTGCTGACCC TCTGTCCCTA CTTGCCAACT TTCTTGTACA 481 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 541 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 601 AGGACGACTT AACGTGAATC TACTACTTAG CACGCGTTAA GTCgacaatc aacctctgga 661 ttacaaaatt tgtgaaagat t