Construct: ORF TRCN0000475677
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001741.1_s317c1
- Derived from:
- ccsbBroadEn_04217
- DNA Barcode:
- ATGTGTATCGAGCCACTTTTTTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TTYH3 (80727)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475677
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80727 | TTYH3 | tweety family member 3 | NM_025250.3 | 100% | 100% | |
| 2 | human | 80727 | TTYH3 | tweety family member 3 | XM_011515535.2 | 94.4% | 94.2% | (many diffs) |
| 3 | human | 80727 | TTYH3 | tweety family member 3 | XM_017012656.1 | 93.8% | 93.8% | 625_626ins96 |
| 4 | human | 80727 | TTYH3 | tweety family member 3 | XM_017012655.1 | 88.5% | 88.2% | (many diffs) |
| 5 | mouse | 78339 | Ttyh3 | tweety family member 3 | NM_175274.5 | 88.8% | 94.6% | (many diffs) |
| 6 | mouse | 78339 | Ttyh3 | tweety family member 3 | XM_017321177.1 | 83.9% | 89.2% | (many diffs) |
| 7 | mouse | 78339 | Ttyh3 | tweety family member 3 | NM_001308040.1 | 80.8% | 85.6% | (many diffs) |
| 8 | mouse | 78339 | Ttyh3 | tweety family member 3 | XM_006504769.3 | 78.4% | 80.5% | (many diffs) |
| 9 | mouse | 78339 | Ttyh3 | tweety family member 3 | XM_006504772.3 | 75.3% | 76.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1638
- ORF length:
- 1569
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggccggggtc agctacgcgg cgccctggtg ggtgagcctc ctgcaccggc 121 tgccccactt cgacctgagc tgggaggcca ctagcagcca gttccggccc gaggacaccg 181 actaccagca ggccctgctg ctcctggggg ccgccgccct ggcctgcctc gccctggacc 241 tcctcttcct gctcttctac tccttctggc tgtgctgccg gcggcgcaag agcgaggagc 301 acctggacgc cgactgctgc tgcacggcct ggtgtgtcat catcgccacg ctggtgtgca 361 gcgccggcat cgcagtggga ttctacggca acggggagac cagtgatggc atccataggg 421 ccacctactc gctccgccac gccaaccgca cggtggccgg ggtccaggac cgcgtgtggg 481 acacggcggt ggggctgaac cacacggcgg agcccagcct gcagaccctg gagcggcagc 541 tggccgggcg gcccgagccc ctgcgagccg tacagaggct gcagggcctg ctggagacgc 601 tgctgggcta cacggccgcc atcccctttt ggaggaacac ggcggtgtcg ctggaggtgc 661 tggcggagca ggtggatctc tacgactggt acaggtggct gggctacctg ggcctgctgc 721 tgctggacgt catcatctgc ctcctggtgc tggttggcct catccgcagc tccaagggca 781 tcctggtggg ggtctgcctg ctgggagtcc tggccctggt catcagctgg ggcgcgctgg 841 gcttggagct ggctgtgtcc gtgggctcca gcgacttctg tgtggaccct gacgcctacg 901 tgaccaaaat ggtggaggag tactcggtgc tgagtgggga catcctgcag tactacctgg 961 cctgctcgcc ccgcgccgcc aaccccttcc agcagaagct gtcgggcagc cacaaggcac 1021 tggtggagat gcaggatgtc gtggctgagc ttctgaggac cgtcccctgg gagcagccgg 1081 ccactaagga ccccctcctc cgcgtccagg aggtgctgaa tggcacggag gtgaacctgc 1141 agcacctcac cgccctggtg gactgccgca gcctgcatct ggactacgtg caagcgctga 1201 ccggcttctg ctatgacggc gtggagggcc tcatctacct ggccctcttc tccttcgtca 1261 cagccctcat gttcagctcc atcgtctgca gcgtcccgca cacctggcag caaaagagag 1321 gcccTGATGA GGACGGGGAG GAGGAGGCCG CTCCAGGGCC GCGGCAGGCG CACGACAGCC 1381 TCTACCGCGT CCACATGCCC AGCCTGTACA GCTGTGGCAG CAGCTACGGC AGTGAGACCA 1441 GCATCCCGGC CGCGGCCCAC ACCGTCAGCA ACGCCCCGGT CACTGAGTAC ATGAGCCAGA 1501 ACGCTAATTT CCAGAACCCC CGCTGTGAGA ACACCCCACT CATTGGGCGC GAGTCCCCGC 1561 CGCCCTCATA CACCTCCAGC ATGAGAGCCA AATACCTCGC CACGAGCCAG CCTCGCCCTG 1621 ACTCCAGCGG CAGCCACTTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1681 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1741 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAATGTGTA TCGAGCCACT 1801 TTTTTACACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt