Construct: ORF TRCN0000475686
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012460.1_s317c1
- Derived from:
- ccsbBroadEn_09595
- DNA Barcode:
- CCTTTATTCTAGGCGGACCCGCAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SMCR8 (140775)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475686
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 140775 | SMCR8 | SMCR8-C9orf72 complex subunit | NM_144775.3 | 99.9% | 99.7% | 1398C>N;1517C>N |
| 2 | mouse | 237782 | Smcr8 | Smith-Magenis syndrome chro... | NM_001085440.1 | 85.8% | 89.5% | (many diffs) |
| 3 | mouse | 237782 | Smcr8 | Smith-Magenis syndrome chro... | NM_175491.4 | 72.2% | 75% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2880
- ORF length:
- 2811
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gatcagcgcc cctgacgtag tggccttcac caaagaggaa gagtatgaag 121 aagagcctta caatgagccg gccctgcctg aggagtactc ggtgccgctc ttccccttcg 181 ccagtcaggg tgctaacccc tggtcaaaac tgtccggggc caagttttcg agggacttca 241 ttcttatttc cgagttctct gagcaggtgg gaccccaacc cttactgacc atccccaatg 301 acaccaaagt ttttggcact tttgatctca attacttctc cctgcgtatc atgtctgtgg 361 attaccaggc ttccttcgtg ggccatcctc ctggatctgc ctaccccaag ctgaacttcg 421 tggaggactc caaggtggtg ctgggagatt ctaaggaggg cgcctttgca tacgtgcacc 481 accttaccct atacgacctg gaggcccgtg gcttcgtgag gccgttttgc atggcttata 541 tctctgcaga ccagcataaa atcatgcagc agttccagga gctttcagcc gaattttcca 601 gagcttctga gtgcttgaag actggcaaca ggaaggcatt tgctggggaa cttgaaaaaa 661 agctgaaaga cttggattac accaggacag tgctacacac agaaacggag atccagaaga 721 aagccaacga caaaggcttt tactcatctc aggcaattga gaaagccaat gaactggcca 781 gtgtggagaa gtccatcatt gaacatcaag acctgctgaa gcagatccgc tcataccctc 841 atcggaagtt gaaggggcat gatttgtgtc ctggtgagat ggagcacatc caggatcagg 901 ccagccaggc atccactacc tctaaccctg atgagtctgc cgacacagac ctttacacct 961 gcagaccagc ctacacccca aaacttatca aagcaaagtc caccaagtgt tttgacaaga 1021 agttgaagac cttggaggag ctctgtgaca ctgaatattt cacccagacc ctggctcagc 1081 tcagccacat tgaacacatg ttcagaggag acctgtgtta cctcctgacc agtcagattg 1141 atagagcact tctaaaacaa cagcatataa caaactttct ctttgaagac tttgtggagg 1201 tcgatgacag gatggtggag aaacaagaaa gcataccctc taagcccagt caagacaggc 1261 cgccttccag ttctctagaa gaatgcccaa ttcctaaagt gttaattagt gttggttctt 1321 acaagtccag tgtggagtct gtgttgatca agatggagca ggaactggga gatgaggagt 1381 acaaggaagt ggaagtgact gagttgagca gtttcgaccc ccaggaaaac ttggactacc 1441 tggatatgga tatgaaaggg agtatnagca gtggtgaaag tattgaagtt ttgggcacgg 1501 agaaatccac ctccgtgctt tctaaatctg acagccaggc aagcctcaca gtaccattga 1561 gcccccaggt ggtccggagc aaagnagtca gccacaggac catcagcgag gacagtattg 1621 aagtcctcag tacctgcccc tctgaggccc tcatccctga tgactttaag gccagctacc 1681 caagtgccat taatgaagaa gaatcatatc cagatggcaa tgaaggagcc atccgcttcc 1741 aggcaagcat cagtcctcca gaactgggtg agacagagga aggcagcata gaaaacaccc 1801 catcacaaat agactcctcc tgctgtattg ggaaggagag cgatggtcag ttggtgctgc 1861 cctccactcc agcccacaca cactctgacg aggatggggt ggtgagcagc cccccacagc 1921 gccacaggca gaaggaccag gggttccgtg tagacttttc agtggaaaat gccaaccctt 1981 cttcccgaga caacagttgt gaagggtttc ccgcttatga gctggacccg agccacctgc 2041 tggctagccg ggacatcagt aagaccagcc tggacaacta ctcagacacc accagctacg 2101 tgagcagtgt agcgtccacc agctcagaca ggatcccctc tgcttatcct gctggcctgt 2161 cttccgatag gcataaaaag agggctggcc agaacgcctt aaaattcatc cgccagtacc 2221 cctttgccca cccagccatc tactccctgc tcagtgggag gacacttgtg gtcctggggg 2281 aagatgaggc catagtcagg aaactcgtga ctgcactggc tatctttgtc cccagctatg 2341 gctgctacgc taagcccgtg aaacattggg cctcctcccc tttgcacatt atggattttc 2401 agaagtggaa gcttattggc ttgcagagag tggcctcccc tgccggtgcc ggtaccctcc 2461 atgccctgag ccgctacagc cgctacacga gcatcctgga ccttgacaac aaaaccctgc 2521 gctgccccct ttacagaggc accctggtgc cccgcctggc agaccaccgc acacagatca 2581 agcggggcag cacctactac ctgcatgtcc agagcatgct cacccagcTC TGCTCCAAGG 2641 CCTTCCTCTA CACCTTCTGC CACCACCTGC ACCTGCCTAC CCACGACAAG GAGACAGAGG 2701 AGCTGGTAGC CAGCCGCCAG ATGAGCTTCC TAAAGCTGAC CCTGGGTCTG GTGAATGAGG 2761 ATGTTAGGGT GGTCCAGTAC CTGGCTGAGC TGCTGAAGCT GCACTACATG CAGGAATCTC 2821 CAGGGACCAG CCACCCCATG CTCAGGTTTG ACTATGTCCC CAGCTTTTTG TATAAAATCT 2881 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 2941 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 3001 TTTATATATC TTGTGGAAAG GACGACCTTT ATTCTAGGCG GACCCGCATA CGCGTTAAGT 3061 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt