Construct: ORF TRCN0000475720
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014232.1_s317c1
- Derived from:
- ccsbBroadEn_09148
- DNA Barcode:
- CCCACTGAACTATGACGCACAATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- USP44 (84101)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475720
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84101 | USP44 | ubiquitin specific peptidas... | NM_001042403.3 | 99.8% | 99.8% | 271A>G;870G>A;1989T>C |
| 2 | human | 84101 | USP44 | ubiquitin specific peptidas... | NM_001278393.2 | 99.8% | 99.8% | 271A>G;870G>A;1989T>C |
| 3 | human | 84101 | USP44 | ubiquitin specific peptidas... | NM_001347936.2 | 99.8% | 99.8% | 271A>G;870G>A;1989T>C |
| 4 | human | 84101 | USP44 | ubiquitin specific peptidas... | NM_001347937.2 | 99.8% | 99.8% | 271A>G;870G>A;1989T>C |
| 5 | human | 84101 | USP44 | ubiquitin specific peptidas... | NM_032147.5 | 99.8% | 99.8% | 271A>G;870G>A;1989T>C |
| 6 | human | 84101 | USP44 | ubiquitin specific peptidas... | XM_017020010.2 | 99.8% | 99.8% | 271A>G;870G>A;1989T>C |
| 7 | human | 84101 | USP44 | ubiquitin specific peptidas... | XM_005269172.1 | 95.6% | 95.5% | (many diffs) |
| 8 | human | 84101 | USP44 | ubiquitin specific peptidas... | XM_005269173.1 | 95.6% | 95.5% | (many diffs) |
| 9 | human | 84101 | USP44 | ubiquitin specific peptidas... | XM_005269174.1 | 95.6% | 95.5% | (many diffs) |
| 10 | human | 84101 | USP44 | ubiquitin specific peptidas... | XM_011538800.2 | 95.6% | 95.5% | (many diffs) |
| 11 | human | 84101 | USP44 | ubiquitin specific peptidas... | XM_011538801.1 | 95.6% | 95.5% | (many diffs) |
| 12 | human | 84101 | USP44 | ubiquitin specific peptidas... | XM_011538802.1 | 95.6% | 95.5% | (many diffs) |
| 13 | human | 84101 | USP44 | ubiquitin specific peptidas... | XM_011538803.1 | 95.6% | 95.5% | (many diffs) |
| 14 | human | 84101 | USP44 | ubiquitin specific peptidas... | XM_011538804.2 | 95.6% | 95.5% | (many diffs) |
| 15 | human | 84101 | USP44 | ubiquitin specific peptidas... | XM_011538805.2 | 95.6% | 95.5% | (many diffs) |
| 16 | human | 84101 | USP44 | ubiquitin specific peptidas... | XM_017020012.1 | 78.6% | 76.1% | (many diffs) |
| 17 | human | 84101 | USP44 | ubiquitin specific peptidas... | XR_001748887.1 | 77.3% | (many diffs) | |
| 18 | human | 84101 | USP44 | ubiquitin specific peptidas... | XR_944757.1 | 77% | (many diffs) | |
| 19 | human | 84101 | USP44 | ubiquitin specific peptidas... | XM_011538806.2 | 69.7% | 68.2% | (many diffs) |
| 20 | human | 84101 | USP44 | ubiquitin specific peptidas... | NR_144945.2 | 52.7% | (many diffs) | |
| 21 | human | 84101 | USP44 | ubiquitin specific peptidas... | NR_144946.2 | 51.6% | (many diffs) | |
| 22 | human | 84101 | USP44 | ubiquitin specific peptidas... | NR_125360.3 | 49.8% | (many diffs) | |
| 23 | human | 84101 | USP44 | ubiquitin specific peptidas... | NR_144948.2 | 49.6% | (many diffs) | |
| 24 | human | 84101 | USP44 | ubiquitin specific peptidas... | NR_144947.2 | 47.6% | (many diffs) | |
| 25 | human | 84101 | USP44 | ubiquitin specific peptidas... | NR_144944.2 | 47.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2202
- ORF length:
- 2136
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct agcaatggat acgtgcaaac atgttgggca gctgcagctt gctcaagacc 121 attccagcct caaccctcag aaatggcact gtgtggactg caacacgacc gagtccattt 181 gggcttgcct tagctgctcc catgttgcct gtggaagata tattgaagag catgcactca 241 agcactttca agaaagcagt catcctgttg cattggaggt gaatgagatg tacgtttttt 301 gttacctttg tgatgattat gttctgaatg ataacgcaac tggagacctg aagttactac 361 gacgtacatt aagtgccatc aaaagtcaaa attatcactg cacaactcgt agtgggaggt 421 ttttacggtc catgggtaca ggtgatgatt cttatttctt acatgacggt gcccaatctc 481 tgcttcaaag tgaagatcaa ctgtatactg ctctttggca caggagaagg atactaatgg 541 gtaaaatctt tcgaacatgg tttgaacaat cacccattgg aagaaaaaag caagaagaac 601 catttcagga aaaaatagta gtaaaaagag aagtaaagaa aagacggcag gaattggagt 661 atcaagttaa agcagaattg gaaagtatgc ctccaagaaa gagtttacgt ttacaagggc 721 tcgctcagtc gaccataata gaaatagttt ctgttcaggt gccagcacaa acgccagcat 781 caccagcaaa agataaagta ctctctacct cagaaaatga aatatctcaa aaagtcagtg 841 actcctcagt taaacgaagg ccaatagtaa ctcctggtgt aacaggattg agaaatttgg 901 gaaatacttg ctatatgaat tctgttcttc aggtattgag tcatttactt atttttcgac 961 aatgtttttt aaagcttgat ctgaaccaat ggctggctat gactgctagc gagaagacaa 1021 gatcttgtaa gcatccacca gtcacagata cagtagtata tcaaatgaat gaatgtcagg 1081 aaaaagatac aggttttgtt tgctccagac aatcaagtct gtcatcagga ctaagtggtg 1141 gagcatcaaa aggtagaaag atggaactta ttcagccaaa ggagccaact tcacagtaca 1201 tttctctttg tcatgaattg catactttgt tccaagtcat gtggtctgga aagtgggcgt 1261 tggtctcacc atttgctatg ctacactcag tgtggagact cattcctgcc tttcgtggtt 1321 acgcccaaca agacgctcag gaatttcttt gtgaactttt agataaaata caacgtgaat 1381 tagagacaac tggtaccagt ttaccagctc ttatccccac ttctcaaagg aaactcatca 1441 aacaagttct gaatgttgta aataacattt ttcatggaca acttcttagt caggttacat 1501 gtcttgcatg tgacaacaaa tcaaatacca tagaaccttt ctgggacttg tcattggagt 1561 ttccagaaag gtatcaatgc agtggaaaag atattgcttc ccagccatgt ctggttactg 1621 aaatgttggc caaatttaca gaaactgaag ctttagaagg aaaaatctac gtatgtgacc 1681 agtgtaactc aaagcgtaga aggttttcct ccaaaccagt tgtactcaca gaagcccaga 1741 aacaacttat gatatgccac ctacctcagg ttctcagact gcacctcaaa cgattcaggt 1801 ggtcaggacg taataaccga gagaagattg gtgttcatgt tggctttgag gaaatcttaa 1861 acatggagcc ctattgctgc agggagaccc tgaaatcccT CAGACCAGAA TGCTTTATCT 1921 ATGACTTGTC CGCGGTGGTG ATGCACCATG GGAAAGGATT TGGCTCAGGG CACTACACTG 1981 CCTACTGCTA TAATTCTGAA GGAGGGTTCT GGGTACACTG CAATGATTCC AAACTAAGCA 2041 TGTGCACTAT GGACGAAGTA TGCAAGGCTC AAGCTTATAT CTTGTTTTAT ACCCAACGAG 2101 TTACTGAGAA TGGACATTCT AAACTTTTGC CTCCAGAGCT CCTGTTGGGG AGCCAACATC 2161 CCAATGAAGA CGCTGATACC TCGTCTAATG AAATCCTTAG CTGCCCAACT TTCTTGTACA 2221 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 2281 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 2341 AGGACGACCC ACTGAACTAT GACGCACAAT GACGCGTTAA GTCgacaatc aacctctgga 2401 ttacaaaatt tgtgaaagat t