Construct: ORF TRCN0000475747

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005945.1_s317c1
Derived from:
ccsbBroadEn_16155
DNA Barcode:
GGGGCGCCCCATCCTTCAAATCAG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
KRTAP19-4 (337971)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000475747
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 337971 KRTAP19-4 keratin associated protein ... NM_181610.3 100% 100%
2 human 337974 KRTAP19-7 keratin associated protein ... NM_181614.2 64.1% 57.4% (many diffs)
3 human 337973 KRTAP19-6 keratin associated protein ... NM_181612.3 60.7% 53.5% (many diffs)
4 human 337972 KRTAP19-5 keratin associated protein ... NM_181611.3 58% 54.8% (many diffs)
5 human 337970 KRTAP19-3 keratin associated protein ... NM_181609.4 56.6% 52.9% (many diffs)
6 mouse 170654 Krtap19-4 keratin associated protein ... NM_130873.1 51.1% 49.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
93
ORF end:
345
ORF length:
252
Sequence:
1TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT
61GATCAACAAG TTTGTACAAA AAAGTTGGCA CCATGAGCTA CTATGGCAGC TATTACAGAG
121GCCTGGGCTA TGGCTGTGGA GGCTTTGGTG GCCTAGGCTA TGGCTATGGC TGTGGATGTG
181GCAGCTTCCG CAGACTGGGT TATGGCTGTG GCTTTGGAGG CAACGGATAT GGCTACTGCC
241GCCCATCATG CTATGGAGGA TATGGATTCT CAATTCTACT GAAATCCTAC CCTGAGGACA
301CGATATCAGA GGTCATAAGA AGATCATTCA ATTTAACCAA ATATTTGCCA ACTTTCTTGT
361ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT
421AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG
481GAAAGGACGA GGGGCGCCCC ATCCTTCAAA TCAGACGCGT TAAGTCgaca atcaacctct
541ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)