Construct: ORF TRCN0000475749
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005491.1_s317c1
- Derived from:
- ccsbBroadEn_10950
- DNA Barcode:
- GTTTCGTATCGCCGAATCCCTTGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LMX1B (4010)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475749
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4010 | LMX1B | LIM homeobox transcription ... | NM_002316.4 | 94% | 94.1% | 1_69del;441A>G;726G>C |
| 2 | human | 4010 | LMX1B | LIM homeobox transcription ... | NM_001174147.2 | 92.3% | 92.5% | (many diffs) |
| 3 | human | 4010 | LMX1B | LIM homeobox transcription ... | NM_001174146.2 | 91.4% | 91.6% | (many diffs) |
| 4 | mouse | 16917 | Lmx1b | LIM homeobox transcription ... | NM_010725.2 | 93.5% | 100% | (many diffs) |
| 5 | mouse | 16917 | Lmx1b | LIM homeobox transcription ... | XM_006497746.3 | 85.2% | 91.1% | (many diffs) |
| 6 | mouse | 16917 | Lmx1b | LIM homeobox transcription ... | XM_006497747.3 | 67.1% | 73% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1182
- ORF length:
- 1116
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt ggacggcatc aagatggagg agcacgccct gcgccccggg cccgccactc 121 tgggggtgct gctgggctcc gactgcccgc atcccgccgt ctgcgagggc tgccagcggc 181 ccatctccga ccgcttcctg atgcgagtca acgagtcgtc ctggcacgag gagtgtttgc 241 agtgcgcggc gtgtcagcaa gccctcacca ccagctgcta cttccgggat cggaaactgt 301 actgcaaaca agactaccaa cagctcttcg cggccaagtg cagcggctgc atggagaaga 361 tcgcccccac cgagttcgtg atgcgggcgc tggagtgcgt gtaccacctg ggctgcttct 421 gctgctgcgt gtgtgagcgg cagctacgca agggcgacga attcgtgctc aaggagggcc 481 agctgctgtg caagggtgac tacgagaagg agaaggacct gctcagctcc gtgagccccg 541 acgagtccga ctccgtgaag agcgaggatg aagatgggga catgaagccg gccaaggggc 601 agggcagtca gagcaagggc agcggggatg acgggaagga cccgcggagg cccaagcgac 661 cccggaccat cctcaccacg cagcagcgaa gagccttcaa ggcctccttc gaggtctcgt 721 ccaagccttg ccgaaaggtc cgagagacac tggcagctga gacgggccTC AGTGTGCGCG 781 TGGTCCAGGT CTGGTTTCAG AACCAAAGAG CAAAGATGAA GAAGCTGGCG CGGCGGCACC 841 AGCAGCAGCA GGAGCAGCAG AACTCCCAGC GGCTGGGCCA GGAGGTCCTG TCCAGCCGCA 901 TGGAGGGCAT GATGGCTTCC TACACGCCGC TGGCCCCACC ACAGCAGCAG ATCGTGGCCA 961 TGGAACAGAG CCCCTACGGC AGCAGCGACC CCTTCCAGCA GGGCCTCACG CCGCCCCAAA 1021 TGCCAGGGAA CGACTCCATC TTCCATGACA TCGACAGCGA TACCTCCTTA ACCAGCCTCA 1081 GCGACTGCTT CCTCGGCTCC TCAGACGTGG GCTCCCTGCA GGCCCGCGTG GGGAACCCCA 1141 TCGACCGGCT CTACTCCATG CAGAGTTCCT ACTTCGCCTC CTGCCCAACT TTCTTGTACA 1201 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1261 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1321 AGGACGAGTT TCGTATCGCC GAATCCCTTG TACGCGTTAA GTCgacaatc aacctctgga 1381 ttacaaaatt tgtgaaagat t