Transcript: Mouse XM_006497747.3

PREDICTED: Mus musculus LIM homeobox transcription factor 1 beta (Lmx1b), transcript variant X2, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Lmx1b (16917)
Length:
4770
CDS:
245..1099

Additional Resources:

NCBI RefSeq record:
XM_006497747.3
NBCI Gene record:
Lmx1b (16917)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006497747.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000070595 TCAGAACCAAAGAGCAAAGAT pLKO.1 691 CDS 100% 5.625 3.938 N Lmx1b n/a
2 TRCN0000429999 AGAGCTTTCAAGGCATCCTTT pLKO_005 584 CDS 100% 4.950 3.465 N Lmx1b n/a
3 TRCN0000414492 TCGGAAGGTCCGAGAGACATT pLKO_005 625 CDS 100% 4.950 3.465 N Lmx1b n/a
4 TRCN0000070593 TGTGTACCACTTGGGCTGTTT pLKO.1 292 CDS 100% 4.950 3.465 N Lmx1b n/a
5 TRCN0000070596 CAGAGTAAAGGCAGTGGAGAT pLKO.1 503 CDS 100% 4.050 2.835 N Lmx1b n/a
6 TRCN0000070594 GCTGCTGTGCAAGGGTGACTA pLKO.1 376 CDS 100% 1.650 1.155 N Lmx1b n/a
7 TRCN0000017514 GAACCAAAGAGCAAAGATGAA pLKO.1 694 CDS 100% 4.950 2.970 N LMX1B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006497747.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10950 pDONR223 100% 67.1% 73% None (many diffs) n/a
2 ccsbBroad304_10950 pLX_304 0% 67.1% 73% V5 (many diffs) n/a
3 TRCN0000475749 GTTTCGTATCGCCGAATCCCTTGT pLX_317 37.3% 67.1% 73% V5 (many diffs) n/a
4 ccsbBroadEn_10951 pDONR223 100% 66.9% 73% None (many diffs) n/a
5 ccsbBroad304_10951 pLX_304 0% 66.9% 73% V5 (many diffs) n/a
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