Construct: ORF TRCN0000475820

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002011.1_s317c1
Derived from:
ccsbBroadEn_09366
DNA Barcode:
ATTCCTTCCGGTGTATCAGCCGTT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SFXN5 (94097)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000475820
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 94097 SFXN5 sideroflexin 5 NM_144579.3 99.9% 100% 522C>T
2 human 94097 SFXN5 sideroflexin 5 NM_001371738.1 93.7% 84.8% 0_1ins61;107_108insGA;459C>T
3 human 94097 SFXN5 sideroflexin 5 NM_001371739.1 93.1% 93.2% 100_101ins69;453C>T
4 human 94097 SFXN5 sideroflexin 5 NM_001330400.2 92.1% 90.4% (many diffs)
5 human 94097 SFXN5 sideroflexin 5 NM_001371737.1 89.1% 83.6% 0_1ins61;109_163del;516C>T
6 human 94097 SFXN5 sideroflexin 5 NM_001330406.2 84.3% 84% 0_1ins112;58_112del;465C>T
7 human 94097 SFXN5 sideroflexin 5 NM_001371740.1 84.3% 72.1% 522C>T;741_742ins86;861_862ins73
8 human 94097 SFXN5 sideroflexin 5 NM_001330402.2 82.5% 81.4% 522C>T;827_828ins118;843_844ins59
9 human 94097 SFXN5 sideroflexin 5 NM_001371741.1 76.9% 71.1% 522C>T;625_626ins116;711_712ins118
10 human 94097 SFXN5 sideroflexin 5 NM_001330401.2 74.3% 56% 522C>T;625_626ins202;759_760ins59
11 human 94097 SFXN5 sideroflexin 5 NM_001330404.2 69.3% 69.4% 0_1ins312;210C>T
12 human 94097 SFXN5 sideroflexin 5 NM_001330410.2 69.3% 69.4% 0_1ins312;210C>T
13 human 94097 SFXN5 sideroflexin 5 NM_001330405.2 67.9% 66% (many diffs)
14 human 94097 SFXN5 sideroflexin 5 NM_001330407.2 63.2% 61.3% (many diffs)
15 human 94097 SFXN5 sideroflexin 5 NM_001371742.1 61.3% 61.1% 522C>T;625_626insCCAGTGCCA;627_628ins384
16 human 94097 SFXN5 sideroflexin 5 NM_001330403.2 60% 42.7% (many diffs)
17 human 94097 SFXN5 sideroflexin 5 NM_001371743.1 59.6% 59.7% 522C>T;534_535ins411
18 human 94097 SFXN5 sideroflexin 5 NM_001330411.2 55.4% 42.4% 0_1ins430;92C>T;170_171ins23
19 human 94097 SFXN5 sideroflexin 5 NM_001330412.2 55.4% 42.4% 0_1ins430;92C>T;170_171ins23
20 human 94097 SFXN5 sideroflexin 5 NM_001330408.2 51.9% 50.8% (many diffs)
21 human 94097 SFXN5 sideroflexin 5 NR_138476.2 23.6% (many diffs)
22 mouse 94282 Sfxn5 sideroflexin 5 NM_178639.4 89.7% 97% (many diffs)
23 mouse 94282 Sfxn5 sideroflexin 5 XM_006506816.4 84.8% 91.4% (many diffs)
24 mouse 94282 Sfxn5 sideroflexin 5 XM_030255684.1 59.5% 64.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1089
ORF length:
1020
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggcggataca gcgactacag catcggcggc ggcggctagt gccgctagcg
121cctcgagcga tgcacctcct ttccaactgg gcaaaccccg cttccagcag acgtccttct
181atggccgctt caggcacttc ttggatatca tcgaccctcg cacactcttt gtcactgaga
241gacgtctcag agaggctgtg cagctgctgg aggactataa gcatgggacc ctgcgcccgg
301gggtcaccaa tgaacagctc tggagtgcac agaaaatcaa gcaggctatt ctacatccgg
361acaccaatga gaagatcttc atgccattta gaatgtcagg ttatattcct tttgggacgc
421caattgtagt cggtcttctc ttgcccaacc agacactggc atccactgtc ttctggcagt
481ggctgaacca gagccacaat gcctgtgtca actatgcaaa ccgcaatgcg accaagcctt
541cacctgcatc caagttcatc cagggatacc tgggagctgt catcagcgct gtctccattg
601ctgtgggcct taatgtcctg gttcagaaag ccaacaagtt caccccagcc acccgccttc
661tcatccagag gtttgtgccg ttccctgctg tagccagtgc caatatctgc aatgtggtcc
721tgatgcggta cggggagctg gaggaaggga ttgatgtcct ggacagcgat ggcaacctcg
781tgggctcctc caagatcgca gcccgacacg ccctgctgga gacggcgctg acgcgagtgg
841tcctgcccat gcccatcctg gtgctacccc cgatcgtcat gtccatgctg gagaagacgg
901ctctcctgca ggcacgcccc cggctgctcc tccctgtgca aagcctcGTG TGCCTGGCAG
961CCTTCGGCCT GGCCCTGCCG CTGGCCATCA GCCTCTTCCC GCAAATGTCA GAGATTGAAA
1021CATCCCAATT AGAGCCGGAG ATAGCCCAGG CCACGAGCAG CCGGACAGTG GTGTACAACA
1081AGGGGTTGTT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC
1141CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA
1201TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAATTCCT TCCGGTGTAT CAGCCGTTAC
1261GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt

Download FASTA (ORF) (Full)