Construct: ORF TRCN0000475848
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016736.1_s317c1
- Derived from:
- ccsbBroadEn_10268
- DNA Barcode:
- TATACATACCTCTTGTATGGTGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DSCR8 (84677)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475848
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84677 | DSCR8 | Down syndrome critical regi... | NR_026841.1 | 48% | 1_171del;445_568del | |
| 2 | human | 84677 | DSCR8 | Down syndrome critical regi... | NR_026840.1 | 46.5% | 1_171del;252_269del;463_586del | |
| 3 | human | 84677 | DSCR8 | Down syndrome critical regi... | NR_026839.1 | 33.4% | (many diffs) | |
| 4 | human | 84677 | DSCR8 | Down syndrome critical regi... | NR_026838.1 | 33% | 1_171del;252_508del;702_825del | |
| 5 | human | 84677 | DSCR8 | Down syndrome critical regi... | NR_026842.1 | 30.9% | 1_171del;251_252ins97;348_471del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 342
- ORF length:
- 273
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttGGCACCAT GAAGGAGCCT GGACCCAACT TTGTTACTGT GAGAAAGGGT CTTCATTCAT 121 TCAAGATGGC ATTTGTTAAG CACCTACTGC TGGAGTGCAG TGGTTCAATC ACGGATCACT 181 GCAGCCTCCA CCTCCCAGTT CAAGAAATTC TCATGTCTCA GCCTCCTGAG CAGCTAGGAT 241 TACAGACAAA CCTTGGAAAT CAAGAAAGTT CTGGAATGAT GAAGCTGTTC ATGCCAAGAC 301 CGAAAGTGCT GGCCCAGTAT GAGTCCATTC AGTTCATGCC GTTGCCAACT TTCTTGTACA 361 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 421 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 481 AGGACGATAT ACATACCTCT TGTATGGTGA CACGCGTTAA GTCgacaatc aacctctgga 541 ttacaaaatt tgtgaaagat t