Construct: ORF TRCN0000475849
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001680.1_s317c1
- Derived from:
- ccsbBroadEn_07515
- DNA Barcode:
- AACATACTTGAACTCCCCCTCGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GFPT2 (9945)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475849
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9945 | GFPT2 | glutamine-fructose-6-phosph... | NM_005110.4 | 99.9% | 99.8% | 1411A>G;1908T>C |
| 2 | mouse | 14584 | Gfpt2 | glutamine fructose-6-phosph... | NM_013529.3 | 89.2% | 97.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2112
- ORF length:
- 2046
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg cggaatcttt gcctacatga actacagagt cccccggacg aggaaggaga 121 tcttcgaaac cctcatcaag ggcctgcagc ggctggagta cagaggctac gactcggcag 181 gtgtggcgat cgatgggaat aatcacgaag tcaaagaaag acacattcag ctggtcaaga 241 aaagggggaa agtcaaggct ctcgatgaag aactttacaa acaagacagc atggacttaa 301 aagtggagtt tgagacacac ttcggcattg cccacacgcg ctgggccacc cacggggtcc 361 ccagtgctgt caacagccac cctcagcgct cagacaaagg caacgaattt gttgtcatcc 421 acaatgggat catcacaaat tacaaagatc tgaggaaatt tctggaaagc aaaggctacg 481 agtttgagtc agaaacagat acagagacca tcgccaagct gattaaatat gtgttcgaca 541 acagagaaac tgaggacatt acgttttcaa cgttggtcga gagagtcatt cagcagttgg 601 aaggtgcatt cgcgctggtt ttcaagagtg tccactaccc aggagaagcc gttgccacac 661 ggagaggcag ccccctgctc atcggagtcc ggagcaaata caagctctcc acagaacaga 721 tccctatctt atacaggacg tgcactctgg agaatgtgaa gaatatctgt aagacacgga 781 tgaagaggct ggacagctcc gcctgcctgc atgctgtggg cgacaaggcc gtggaattct 841 tctttgcttc tgatgcaagc gctatcatag agcacaccaa ccgggtcatc ttcctggagg 901 acgatgacat cgccgcagtg gctgatggga aactctccat tcaccgggtc aagcgctcgg 961 ccagtgatga cccatctcga gccatccaga ccttgcagat ggaactgcag caaatcatga 1021 aaggtaactt cagtgcgttt atgcagaagg agatcttcga acagccagaa tcagttttca 1081 atactatgag aggtcgggtg aattttgaaa ccaacacagt gctcctgggt ggcttgaagg 1141 accacttgaa ggagattcga cgatgccgac ggctcatcgt gattggctgt ggaaccagct 1201 accacgctgc cgtggctacg cggcaagttt tggaggaact gactgagctt cctgtgatgg 1261 ttgaacttgc tagtgatttt ctggacagga acacacctgt gttcagggat gacgtttgct 1321 ttttcatcag ccagtcaggc gagaccgcgg acaccctcct ggcgctgcgc tactgtaagg 1381 accgcggcgc tctcaccgtg ggcgtcacca acaccgtggg cagctccatc tctcgcgaga 1441 ccgactgcgg cgtccacatc aacgcagggc cggaggtcgg cgtggccagc accaaggctt 1501 ataccagtca gttcatctct ctggtgatgt ttggtttgat gatgtctgaa gaccgaattt 1561 cactacaaaa caggaggcaa gagatcatcc gtggcttgag atctttacct gagctgatca 1621 aggaagtgct gtctctggag gagaagatcc acgacttggc cctggagctc tacacgcaga 1681 gatcgctgct ggtgatgggg cggggctaca actatgccac ctgcctggaa ggagccctga 1741 aaattaaaga gataacctac atgcactcag aaggcatcct ggctggGGAG CTGAAGCACG 1801 GGCCCCTGGC ACTGATTGAC AAGCAGATGC CCGTCATCAT GGTCATTATG AAGGATCCTT 1861 GCTTCGCCAA ATGCCAGAAC GCCCTGCAGC AAGTCACGGC CCGCCAGGGT CGCCCCATTA 1921 TACTGTGCTC CAAGGACGAT ACTGAAAGTT CCAAGTTTGC GTATAAGACA ATCGAGCTGC 1981 CCCACACTGT GGACTGCCTC CAGGGCATCC TGAGCGTGAT TCCGCTGCAG CTGCTGTCCT 2041 TCCACCTGGC TGTTCTCCGA GGATATGACG TTGACTTCCC CAGAAATCTG GCCAAGTCTG 2101 TAACTGTGGA ATGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 2161 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 2221 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAAAC ATACTTGAAC TCCCCCTCGA 2281 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t