Construct: ORF TRCN0000475888
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001567.1_s317c1
- Derived from:
- ccsbBroadEn_09528
- DNA Barcode:
- CTCTAAATGACAAGACCAGCTCTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CPO (130749)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475888
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 130749 | CPO | carboxypeptidase O | NM_173077.3 | 99.6% | 99.1% | (many diffs) |
| 2 | human | 130749 | CPO | carboxypeptidase O | XM_017003372.2 | 99.6% | 99.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1191
- ORF length:
- 1122
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaagcttctg cttgaaaccc tttatctttt ggggatgctg gttcctggag 121 ggctgggata tgatagatcc ttagcccaac acagacaaga gattgtggac aagtcagtga 181 gtccatggag cctggagacg tattcctata acatatacca ccccatggga gagatctatg 241 agtggatgag agagatcagt gagaagtaca aggaagtggt gacacagcat ttcctaggag 301 tgacctatga gacccacccc atatattatc tgaagatcag ccaaccatct ggtaatccca 361 agaaaatcat ttggatggac tgtggaattc acgccagaga atggattgct cctgcttttt 421 gccaatggtt cgtcaaagaa attctacaaa accataaaga caactcaagg atacgcaagc 481 tccttaggaa cctggacttc tatgtccttc cagttcttaa catagatggt tatatctaca 541 cttggacaac tgatcgtctt tggaggaaat cccgttcacc ccataataat ggcacatgtt 601 ttgggacgga tctcaatcga aatttcaatg cttcttggtg tagtattggt gcctctagaa 661 actgccaaga tcaaacattc tgtgggacag ggccagtgtc tgaaccagag actaaagctg 721 ttgccagctt catagagagc aagaaggatg atattttgtg cttcctgacc atgcactctt 781 atgggcagtt aattctcaca ccttacggct acaccaaaaa taaatcaagt aaccacccag 841 aaatgattca agttGGACAG AAGGCAGCAA ATGCATTGAA AGCAAAGTAT GGAACCAATT 901 ATAGAGTTGG ATCGAGTGCA GATATTTTAT ATGCCTCATC AGGGTCTTCA AGAGATTGGG 961 CCCGAGACAT TGGGATTCCC TTCTCATATA CGTTTGAGCT GAGGGACAGT GGAACATATG 1021 GGTTTGTTCT GCCAGAAGCT CAGATCCAGC CCACCTGTGA GGAGACCATG GAGGCTGTGC 1081 TGTCAGTCCT GGATGATGTG TATGCGAAAC ACTGGCACTC GGACAGTGCT GGAAGGGTGA 1141 CATCTGCCAC TATGCTGCTG GGCCTGCTGG TGTCCTGCAT GTCTCTTCTC TTGCCAACTT 1201 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1261 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1321 CTTGTGGAAA GGACGACTCT AAATGACAAG ACCAGCTCTG ACGCGTTAAG TCgacaatca 1381 acctctggat tacaaaattt gtgaaagatt