Construct: ORF TRCN0000475893
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012569.1_s317c1
- Derived from:
- ccsbBroadEn_00403
- DNA Barcode:
- CACCGTCACGGTGCCGGGGGGCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CYBB (1536)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475893
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1536 | CYBB | cytochrome b-245 beta chain | NM_000397.4 | 100% | 100% | |
| 2 | mouse | 13058 | Cybb | cytochrome b-245, beta poly... | NM_007807.5 | 87.5% | 93.1% | (many diffs) |
| 3 | mouse | 13058 | Cybb | cytochrome b-245, beta poly... | XM_006527565.3 | 87.5% | 93.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1776
- ORF length:
- 1710
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gaactgggct gtgaatgagg ggctctccat ttttgtcatt ctggtttggc 121 tggggttgaa cgtcttcctc tttgtctggt attaccgggt ttatgatatt ccacctaagt 181 tcttttacac aagaaaactt cttgggtcag cactggcact ggccagggcc cctgcagcct 241 gcctgaattt caactgcatg ctgattctct tgccagtctg tcgaaatctg ctgtccttcc 301 tcaggggttc cagtgcgtgc tgctcaacaa gagttcgaag acaactggac aggaatctca 361 cctttcataa aatggtggca tggatgattg cacttcactc tgcgattcac accattgcac 421 atctatttaa tgtggaatgg tgtgtgaatg cccgagtcaa taattctgat ccttattcag 481 tagcactctc tgaacttgga gacaggcaaa atgaaagtta tctcaatttt gctcgaaaga 541 gaataaagaa ccctgaagga ggcctgtacc tggctgtgac cctgttggca ggcatcactg 601 gagttgtcat cacgctgtgc ctcatattaa ttatcacttc ctccaccaaa accatccgga 661 ggtcttactt tgaagtcttt tggtacacac atcatctctt tgtgatcttc ttcattggcc 721 ttgccatcca tggagctgaa cgaattgtac gtgggcagac cgcagagagt ttggctgtgc 781 ataatataac agtttgtgaa caaaaaatct cagaatgggg aaaaataaag gaatgcccaa 841 tccctcagtt tgctggaaac cctcctatga cttggaaatg gatagtgggt cccatgtttc 901 tgtatctctg tgagaggttg gtgcggtttt ggcgatctca acagaaggtg gtcatcacca 961 aggtggtcac tcaccctttc aaaaccatcg agctacagat gaagaagaag gggttcaaaa 1021 tggaagtggg acaatacatt tttgtcaagt gcccaaaggt gtccaagctg gagtggcacc 1081 cttttacact gacatccgcc cctgaggaag acttctttag tatccatatc cgcatcgttg 1141 gggactggac agaggggctg ttcaatgctt gtggctgtga taagcaggag tttcaagatg 1201 cgtggaaact acctaagata gcggttgatg ggccctttgg cactgccagt gaagatgtgt 1261 tcagctatga ggtggtgatg ttagtgggag cagggattgg ggtcacaccc ttcgcatcca 1321 ttctcaagtc agtctggtac aaatattgca ataacgccac caatctgaag ctcaaaaaga 1381 tctacttcta ctggctgtgc cgggacacac atgcctttga gtggtttgca gatctgctgc 1441 aactgctgga gagccagatg caggaaagga acaatgccgg cttccTCAGC TACAACATCT 1501 ACCTCACTGG CTGGGATGAG TCTCAGGCCA ATCACTTTGC TGTGCACCAT GATGAGGAGA 1561 AAGATGTGAT CACAGGCCTG AAACAAAAGA CTTTGTATGG ACGGCCCAAC TGGGATAATG 1621 AATTCAAGAC AATTGCAAGT CAACACCCTA ATACCAGAAT AGGAGTTTTC CTCTGTGGAC 1681 CTGAAGCCTT GGCTGAAACC CTGAGTAAAC AAAGCATCTC CAACTCTGAG TCTGGCCCTC 1741 GGGGAGTGCA TTTCATTTTC AACAAGGAAA ACTTCTACCC AACTTTCTTG TACAAAGTGG 1801 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1861 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1921 ACACCGTCAC GGTGCCGGGG GGCGCACGCG TTAAGTCgac aatcaacctc tggattacaa 1981 aatttgtgaa agatt