Construct: ORF TRCN0000475902
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009886.1_s317c1
- Derived from:
- ccsbBroadEn_06342
- DNA Barcode:
- ACATGTTTTTGACAAGCCGTGGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GUCY1A1 (2982)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475902
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2982 | GUCY1A1 | guanylate cyclase 1 soluble... | NM_000856.6 | 99.7% | 99.4% | (many diffs) |
| 2 | human | 2982 | GUCY1A1 | guanylate cyclase 1 soluble... | NM_001130682.3 | 99.7% | 99.4% | (many diffs) |
| 3 | human | 2982 | GUCY1A1 | guanylate cyclase 1 soluble... | NM_001130683.4 | 99.7% | 99.4% | (many diffs) |
| 4 | human | 2982 | GUCY1A1 | guanylate cyclase 1 soluble... | NM_001130684.3 | 99.7% | 99.4% | (many diffs) |
| 5 | human | 2982 | GUCY1A1 | guanylate cyclase 1 soluble... | NM_001256449.2 | 99.7% | 99.4% | (many diffs) |
| 6 | human | 2982 | GUCY1A1 | guanylate cyclase 1 soluble... | XM_005262955.3 | 99.7% | 99.4% | (many diffs) |
| 7 | human | 2982 | GUCY1A1 | guanylate cyclase 1 soluble... | XM_006714196.2 | 99.7% | 99.4% | (many diffs) |
| 8 | human | 2982 | GUCY1A1 | guanylate cyclase 1 soluble... | XM_006714197.2 | 99.7% | 99.4% | (many diffs) |
| 9 | human | 2982 | GUCY1A1 | guanylate cyclase 1 soluble... | NM_001130687.2 | 90.1% | 90% | (many diffs) |
| 10 | human | 2982 | GUCY1A1 | guanylate cyclase 1 soluble... | NM_001130685.3 | 65.7% | 65.6% | (many diffs) |
| 11 | human | 2982 | GUCY1A1 | guanylate cyclase 1 soluble... | XM_005262956.3 | 65.7% | 65.6% | (many diffs) |
| 12 | human | 2982 | GUCY1A1 | guanylate cyclase 1 soluble... | XM_005262957.3 | 65.7% | 65.6% | (many diffs) |
| 13 | human | 2982 | GUCY1A1 | guanylate cyclase 1 soluble... | XM_011531900.2 | 65.7% | 65.6% | (many diffs) |
| 14 | mouse | 60596 | Gucy1a3 | guanylate cyclase 1, solubl... | NM_021896.5 | 84% | 89.5% | (many diffs) |
| 15 | mouse | 60596 | Gucy1a3 | guanylate cyclase 1, solubl... | XM_006501843.3 | 84% | 89.5% | (many diffs) |
| 16 | mouse | 60596 | Gucy1a3 | guanylate cyclase 1, solubl... | XM_011240184.1 | 84% | 89.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2136
- ORF length:
- 2070
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt ctgcaggaag ctcaaggatc tcaagatcac aggagagtgt cctttctcct 121 tactggcacc aggtcaagtt cctaacgagt cttcagagga ggcagcagga agctcagaga 181 gctgcaaagc aaccatgccc atctgtcaag acattcctga gaagaacata caagaaagtc 241 ttcctcaaag aaaaaccagt cggagccgag tctatcttca cactttggca gagagtattt 301 gcaaactgat tttcccagag tttgaacggc tgaatgttgc acttcagaga acattggcaa 361 agcacaaaat aaaagaaagc aggaaatctt tggaaagaga agactttgaa aaaacaattg 421 cagagcaagc agttgcagca ggagttccag tggaggttat caaagaatct cttggtgaag 481 aggtttttaa aatatgttac gaggaagatg aaaacatcct tggggtggtt ggaggcaccc 541 ttaaagattt tttaaacagc ttcagtaccc ttctgaaaca gagcagccat tgccaagaag 601 caggaaaaag gggcaggctt gaggacgcct ccattctatg cctggataag gaggatgatt 661 ttctacatgt ttactacttc ttccctaaga gaaccacctc cctgattctt cccggcatca 721 taaaggcagc tgctcacgta ttatatgaaa cggaagtgga agtgtcgtta atgcctccct 781 gcttccataa tgattgcagc gagtttgtga atcagcccta cttgttgtac tccgttcaca 841 tgaaaagcac caagccatcc ctgtccccca gcaaacccca gtcctcgctg gtgattccca 901 catcgctatt ctgcaagaca tttccattcc atttcatgtt tgacaaagat atgacaattc 961 tgcaatttgg caatggcatc agaaggctga tgaacaggag agactttcaa ggaaagccta 1021 attttgaaga atactttgaa attctgactc caaaaatcaa ccagacgttt agcgggatca 1081 tgactatgtt gaatatgcag tttgttgtac gagtgaggag atgggacaac tctgtgaaga 1141 aatcttcaag ggttatggac ctcaaaggcc aaatgatcta cattgttgaa tccagtgcaa 1201 tcttgttttt ggggtcaccc tgtgtggaca gattagaaga ttttacagga cgagggctct 1261 acctctcaga catcccaatt cacaatgcac tgagggatgt ggtcttaata ggggaacaag 1321 cccgagctca agatggcctg aagaagaggc tggggaagct gaaggctacc cttgagcaag 1381 cccaccaagc cctggaggag gagaagaaaa agacagtaga ccttctgtgc tccatatttc 1441 cctgtgaggt tgctcagcag ctgtggcaag ggcaagttgt gcaagccaag aagttcagta 1501 atgtcaccat gctcttctca gacatcgttg ggttcactgc catctgctcc cagtgctcac 1561 cgctgcaggt catcaccatg ctcaatgcac tgtacactcg cttcgaccag cagtgtggag 1621 agctggatgt ctacaaggtg gagaccattg gcgatgccta ttgtgtagct gggggattac 1681 acaaagagag tgatactcat gctgttcaga tagcgctgat ggccgtgaag atgatggagc 1741 tctctgatga agttatgtct ccccatggag aacctatcaa gatgcgaatt ggactgcact 1801 ctggatcagt ttttgctggc gtcgttGGAG TTAAAATGCC CCGTTACTGT CTTTTTGGAA 1861 ACAATGTCAC TCTGGCTAAC AAATTTGAGT CCTGCAGTGT ACCACGAAAA ATCAATGTCA 1921 GCCCAACAAC TTACAGATTA CTCAAAGACT GTCCTGGTTT CGTGTTTACC CCTCGATCAA 1981 GGGAGGAACT TCCACCAAAC TTCCCTAGTG AAATCCCCGG AATCTGCCAT TTTCTGGATG 2041 CTTACCAACA AGGAACAAAC TCAAAACCAT GCTTCCAAAA GAAAGATGTG GAAGATGGCA 2101 ATGCCAATTT TTTAGGCAAA GCATCAGGAA TAGAATACCC AACTTTCTTG TACAAAGTGG 2161 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 2221 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 2281 AACATGTTTT TGACAAGCCG TGGTGACGCG TTAAGTCgac aatcaacctc tggattacaa 2341 aatttgtgaa agatt