Construct: ORF TRCN0000475917
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017755.1_s317c1
- Derived from:
- ccsbBroadEn_14501
- DNA Barcode:
- TGCCGGAGTCATCAAACTTAGTCC
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SRRD (402055)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475917
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 402055 | SRRD | SRR1 domain containing | NM_001013694.3 | 97.4% | 96.1% | (many diffs) |
| 2 | human | 402055 | SRRD | SRR1 domain containing | XM_011530178.2 | 78.9% | 76.2% | (many diffs) |
| 3 | human | 402055 | SRRD | SRR1 domain containing | XM_017028799.2 | 64.3% | 58.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1062
- ORF length:
- 996
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgcggccgca gctgcggcgc tggaatcctg gcaggcggcg gctccgcgga 121 agaggcgctc cgcggctcga cggccgcggc ggagggaggc ggcgccccgg gggagagagg 181 cggcgccccg gggccccgag gcggagttag agtctgacag cggagtcgtg cttcgtcgca 241 tctgggaggc tgagaaggac ctgtttatct ctgatttctg gagttcagca ctagaaacca 301 tcaatagatg tctaacaaaa catctggaac aactgaagac ccctgtgggg actctttcag 361 acatctttgg aaacctgcat cttgactcat tgccagagga gtcagatgtg gccactgatt 421 ctatcccaag agagatcttg gtcacaggaa cctgccattt gaagtgtgtg tgttacggca 481 ttgggaactt tgccacctgc atcgtagcta gaaaccagct aacgtttttg ctgcttttgt 541 tggaaaagtg ccagattccc agaagtcact gttgggtata tgaccctctg tttagccaac 601 ttgaaattga agtccttaac acccttggtg tgactgttct cagtgagaac gaggaaggga 661 aacggagtat tcgcggggag cctaccatct tttacatgct ccattgtggg acggccttgt 721 acaacaatct tttatggagt aactggtcag tagatgccct ttctaagatg gtcatcattg 781 ggaacagttt CAAAGGACTT GAGGAGAGGT TGTTGGCAAG GATTCTGCAG AAAAATTATC 841 CCTACATTGC AAAGATTTTA AAAGGACTGG AGGAGCTTGA GTTTCCTCAG ACTTCACAAT 901 ACATGGACAT ATTTAATGAT ACCTCTGTCC ACTGGTTCCC TGTGCAAAAG CTAGAACAGC 961 TCTCCATAGA TATTTGGGAG TTTTGGGAAG AACCAGATTA TCAGGACTGT GAGGACCTTG 1021 AAATCATCAG GAACAAGAGA GAAGATCCTT CTGTACTGAC TGCCCAACTT TCTTGTACAA 1081 AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA 1141 ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA 1201 GGACGATGCC GGAGTCATCA AACTTAGTCC ACGCGTTAAG TCgacaatca acctctggat 1261 tacaaaattt gtgaaagatt