Construct: ORF TRCN0000475922
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013627.1_s317c1
- Derived from:
- ccsbBroadEn_04140
- DNA Barcode:
- TGTCTAGGGGTTATGGAACATGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TCTN2 (79867)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475922
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79867 | TCTN2 | tectonic family member 2 | NM_024809.5 | 100% | 100% | |
| 2 | human | 79867 | TCTN2 | tectonic family member 2 | NM_001143850.3 | 99.8% | 99.8% | 266_267insGAA |
| 3 | human | 79867 | TCTN2 | tectonic family member 2 | XM_006719605.3 | 94.9% | 94.9% | 1986_1986delCins106 |
| 4 | human | 79867 | TCTN2 | tectonic family member 2 | XM_005253623.2 | 93.5% | 93.5% | 1097_1098ins135 |
| 5 | human | 79867 | TCTN2 | tectonic family member 2 | XM_017019974.1 | 93.4% | 93.4% | 266_267insGAA;1094_1095ins135 |
| 6 | human | 79867 | TCTN2 | tectonic family member 2 | XM_017019975.1 | 56.3% | 56.3% | 0_1ins912 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2157
- ORF length:
- 2091
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg cttccagcct ccggccgctc ttcttttgag gcttttcctt ctgcagggca 121 tcctgaggct tctgtggggg gacctggctt tcatccctcc ttttatccga atgtccggcc 181 ctgcggtcag cgcgtccctg gtcggagaca ccgagggtgt gaccgtgtcc ctggcagtgc 241 tgcaggacga ggcgggaata ttgccaattc cgacgtgtgg agtgctgaac aatgagacgg 301 aagactggag cgtgactgtg atccccggtg cgaaggtgtt ggaagtgaca gtgaggtgga 361 agagaggtct ggactggtgt tcctccaatg agacagattc cttctcagag tccccctgta 421 tcctccagac ccttctggtt tcagcatctc ataattcatc ctgttcagca catctactca 481 ttcaagtgga aatttatgcc aactcttctc tgacccataa tgcctcagag aacgtgactg 541 tcattcctaa ccaggtgtat cagccccttg gcccttgtcc ttgtaattta acagctggag 601 cctgtgatgt tcgctgctgc tgtgaccagg aatgctcatc aaatttaaca acgctgttca 661 gacggtcctg cttcaccggc gtgtttggag gagacgtcaa tcctcctttt gatcagctct 721 gctctgctgg gacgacgaca cgtggtgtcc ccgattggtt tccctttctg tgtgtgcagt 781 ccccccttgc caacacaccc ttccttggtt acttctatca tggtgctgtt tcccccaaac 841 aggactcttc ctttgaagta tatgtggata ctgacgcaaa agactttgca gactttggtt 901 acaaacaagg agatcccatt atgactgtaa agaaggcata ttttactatt ccgcaggtgt 961 ccctggctgg gcagtgtatg cagaacgccc cagtggcatt tcttcacaat tttgatgtta 1021 aatgcgttac taatttggaa ctataccaag aacgagatgg tattatcaat gcgaagataa 1081 agaatgttgc cttaggaggc atagttacac caaaagtgat ctatgaggaa gcaactgacc 1141 tagacaaatt catcaccaat acagaaactc ctttaaataa cggatcaacc cctagaattg 1201 tgaatgtgga agaacattat attttcaaat ggaataataa taccatcagt gaaataaatg 1261 ttaaaatttt tagggcagag attaatgccc accagaaagg gataatgaca cagagatttg 1321 tagtaaaatt tttaagctat aatagtggta atgaagaaga attatctgga aatccaggtt 1381 accaacttgg caagcctgtc cgagctctaa atatcaacag gatgaataat gtcacgactt 1441 tacatctttg gcaatcggct ggaaggggtc tgtgtacatc agcaactttc aaacccattt 1501 tatttggaga aaatgtactc tctggatgcc tgttagaagt cgggattaat gaaaattgta 1561 ctcagctcag ggagaatgct gttgaaagac ttgattcatt aatacaagcg actcacgttg 1621 caatgagagg caactccgat tacgctgatc ttagtgatgg ctggctcgaa ataatacgtg 1681 tagatgcccc tgatccaggt gcagacccgc tggctagcag tgtgaacggc atgtgcctgg 1741 atattcctgc tcacctgagc atccgcatcc tcatctcgga tgctggcgcg gtggaaggga 1801 ttactcagca ggagatactc ggtgtagaga caaggttctc cTCAGTGAAC TGGCAGTACC 1861 AGTGTGGGCT TACCTGTGAG CACAAGGCCG ACCTTCTCCC TATCAGTGCA TCCGTCCAGT 1921 TTATTAAAAT TCCTGCACAG TTACCCCACC CCCTGACAAG ATTCCAGATC AATTATACAG 1981 AGTATGACTG CAACAGAAAT GAGGTGTGTT GGCCGCAGCT TCTATATCCA TGGACTCAGT 2041 ATTATCAAGG GGAGCTGCAT TCTCAGTGTG TTGCTAAGGG CTTACTGTTG CTGTTGTTCC 2101 TCACATTGGC CTTGTTCCTC AGCAACCCCT GGACCAGAAT ATGCAAAGCC TATAGTTACC 2161 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 2221 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 2281 ATATATCTTG TGGAAAGGAC GATGTCTAGG GGTTATGGAA CATGTCACGC GTTAAGTCga 2341 caatcaacct ctggattaca aaatttgtga aagatt