Construct: ORF TRCN0000475926
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014203.1_s317c1
- Derived from:
- ccsbBroadEn_06417
- DNA Barcode:
- TCGCAGGTTTGCCACGAATGCCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HTR1E (3354)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475926
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3354 | HTR1E | 5-hydroxytryptamine recepto... | NM_000865.3 | 99.9% | 100% | 531C>T |
| 2 | human | 3354 | HTR1E | 5-hydroxytryptamine recepto... | XM_011535789.2 | 99.9% | 100% | 531C>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1161
- ORF length:
- 1095
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa catcacaaac tgtaccacag aggccagcat ggctataaga cccaagacca 121 tcactgagaa gatgctcatt tgcatgactc tggtggtcat caccaccctc accacgttgc 181 tgaacttggc tgtgatcatg gctattggca ccaccaagaa gctccaccag cctgccaact 241 acctaatctg ttctctggcc gtgacggacc tcctggtggc agtgctcgtc atgcccctga 301 gcatcatcta cattgtcatg gatcgctgga agcttgggta cttcctctgt gaggtgtggc 361 tgagtgtgga catgacctgc tgcacctgct ccatcctcca cctctgtgtc attgccctgg 421 acaggtactg ggccatcacc aatgctattg aatacgccag gaagaggacg gccaagaggg 481 ccgcgctgat gatccttacc gtctggacca tctccatttt catctccatg ccccctctgt 541 tctggagaag ccaccgccgc ctaagccctc cccctagtca gtgcaccatc cagcatgacc 601 atgttatcta caccatttac tccacgctgg gtgcgtttta tatccccttg actttgatac 661 tgattctcta ttaccggatt taccacgcgg ccaagagcct ttaccagaaa aggggatcaa 721 gtcggcactt aagcaacaga agcacagata gccagaattc ttttgcaagt tgtaaactta 781 cacagacttt ctgtgtgtct gacttctcca ccTCAGACCC TACCACAGAG TTTGAAAAGT 841 TCCATGCCTC CATCAGGATC CCCCCCTTCG ACAATGATCT AGATCACCCA GGAGAACGTC 901 AGCAGATCTC TAGCACCAGG GAACGGAAGG CAGCACGCAT CCTGGGGCTG ATTCTGGGTG 961 CATTCATTTT ATCCTGGCTG CCATTTTTCA TCAAAGAGTT GATTGTGGGT CTGAGCATCT 1021 ACACCGTGTC CTCGGAAGTG GCCGACTTTC TGACGTGGCT CGGTTATGTG AATTCTCTGA 1081 TCAACCCTCT GCTCTATACG AGTTTTAATG AAGACTTTAA GCTGGCTTTT AAAAAGCTCA 1141 TTAGATGCCG AGAGCATACT TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1201 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1261 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATCGC AGGTTTGCCA 1321 CGAATGCCTC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt