Construct: ORF TRCN0000475946
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004085.1_s317c1
- Derived from:
- ccsbBroadEn_06633
- DNA Barcode:
- GTACGAGAATTTCTCACTGTAGCC
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NEUROD2 (4761)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475946
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4761 | NEUROD2 | neuronal differentiation 2 | NM_006160.4 | 99.6% | 98.9% | (many diffs) |
| 2 | mouse | 18013 | Neurod2 | neurogenic differentiation 2 | NM_010895.3 | 91.7% | 96.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1212
- ORF length:
- 1146
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gacccgcctg ttcagcgagc ccggccttct ctcggacgtg cccaagttcg 121 ccagctgggg cgacggcgaa gacgacgagc cgaggagcga caagggcgac gcgccgccac 181 cgccaccgcc tgcgcccggg ccaggggctc cggggccagc ccgggcggcc aagccagtcc 241 ctctccgtgg agaagagggg acggaggcca cgttggccga ggtcaaggag gaaggcgagc 301 tggggggaga ggaggaggag gaagaggagg aggaagaagg actggacgag gcggagggcg 361 agcggcccaa gaagggcggg cccaagaagc gcaagatgac caaggcgcgc ttggagcgct 421 ccaagcttcg gcggcagaag gcgaacgcgc gggagcgcaa ccgcatgcac gacctgaacg 481 cagccctgga caacctgcgc aaggtggtgc cctgctactc caagacgcag aagctgtcca 541 agatcgagac gctgcgccta gccaagaact atatctgggc gctctcggag atcctgcgct 601 ccggcaagcg gccagaccta gtgtcctacg tgcagactct gtgcaagggt ctgtcgcagc 661 ccaccaccaa tctggtggcc ggctgtctgc agctcaactc tcgcaacttc ctcacggagc 721 aaggcgccga cggtgccggc cgcttccacg gctcgggcgg cccgttcgcc atgcacccct 781 acccgtaccc gtgctcgcgc ctggcgggcg cacagtgcca ggcggccggc ggcctgggcg 841 gcggcgcggc gcacgccctg cggacccacg gctactgcgc agcctacgag acgctgtatg 901 cggcggcagg cggtggcggc gcgagcccgg actacCACAG CTCCGAGTAC GAGGGCCCGC 961 TCAGCCCCCC GCTCTGTCTC AATGGCAACT TCTCACTCAA GCAGGACTCC TCGCCCGACC 1021 ACGAGAAAAG CTACCACTAC TCTATGCACT ACTCGGCGCT GCCCGGTTCG CGGCCCACGG 1081 GCCACGGGCT AGTCTTCGGC TCGTCGGCTG TGCGCGGGGG CGTCCACTCG GAGAATCTCT 1141 TGTCTTACGA TATGCACCTT CACCACGACC GGGGCCCCAT GTACGAGGAG CTCAATGCGT 1201 TTTTTATAAC TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA 1261 CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC 1321 GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGTAC GAGAATTTCT CACTGTAGCC 1381 ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt