Construct: ORF TRCN0000475974
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004923.1_s317c1
- Derived from:
- ccsbBroadEn_13661
- DNA Barcode:
- TTAAAGTACGGATACTAATTGCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C9orf153 (389766)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475974
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 389766 | C9orf153 | chromosome 9 open reading f... | NM_001276366.2 | 100% | 100% | |
| 2 | human | 389766 | C9orf153 | chromosome 9 open reading f... | NM_001276367.2 | 100% | 100% | |
| 3 | human | 389766 | C9orf153 | chromosome 9 open reading f... | NM_001276368.1 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 354
- ORF length:
- 285
- Sequence:
-
1 tcttccattt CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCACCAT GTTCCTCACT GGAGACACCA GTCCAGCTGA GGACAATAGA GAAGCCACCC 121 TTCCTCAATG TTCACTTCCA GAATTATATG CATGTATTGA GAATTTTAAT AAGGAGAGCA 181 AGAAATCAAA TCTTCTAAAA ATGCATGGTA TTTCACTTAA CGAAGCACAG GAAGTACTTG 241 CTAGAAACCT GAATGTCATG TCATTCACCA GGGGCGCTGA TGTGAGAGGA GATCTCCAAC 301 CTGTTATCAG GAAAACAATT CATGAATCTA AGGGATCTGG AATGGAGATA TTTTTGCCAA 361 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 421 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 481 TATCTTGTGG AAAGGACGAT TAAAGTACGG ATACTAATTG CTAACGCGTT AAGTCgacaa 541 tcaacctctg gattacaaaa tttgtgaaag att