Construct: ORF TRCN0000475984
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012097.1_s317c1
- Derived from:
- ccsbBroadEn_11555
- DNA Barcode:
- CGGTTCTGAACGAAAAGGTCGATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- WASF3 (10810)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475984
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10810 | WASF3 | WASP family member 3 | NM_001291965.1 | 100% | 100% | |
| 2 | human | 10810 | WASF3 | WASP family member 3 | NM_006646.6 | 92.1% | 90.6% | (many diffs) |
| 3 | human | 10810 | WASF3 | WASP family member 3 | XM_011534889.2 | 92.1% | 90.6% | (many diffs) |
| 4 | human | 10810 | WASF3 | WASP family member 3 | XM_011534890.1 | 92.1% | 90.6% | (many diffs) |
| 5 | human | 10810 | WASF3 | WASP family member 3 | XM_024449315.1 | 92.1% | 90.6% | (many diffs) |
| 6 | mouse | 245880 | Wasf3 | WAS protein family, member 3 | XM_006504834.2 | 85% | 92% | (many diffs) |
| 7 | mouse | 245880 | Wasf3 | WAS protein family, member 3 | NM_145155.3 | 79.1% | 84.7% | (many diffs) |
| 8 | mouse | 245880 | Wasf3 | WAS protein family, member 3 | XM_006504833.3 | 79.1% | 84.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1563
- ORF length:
- 1497
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc tttagtgaag aggaacattg agccccggca cttgtgccgg ggagctctgc 121 ctgaagggat taccagcgaa cttgaatgtg taaccaatag tactcttgcc gctatcatac 181 gccagctgag cagtctgagc aaacatgctg aagacatatt tggtgagttg tttaatgagg 241 ctaacaactt ctacatcaga gcaaattctc ttcaagacag aattgatcgc cttgctgtca 301 aagtcaccca gctggattca acagtggaag aggtctcact acaggatatc aacatgaaaa 361 aagctttcaa aagttccaca gtccaagacc agcaagtggt ttcaaagaac agcattccta 421 atcctgttgc tgatatttac aaccagagtg ataagccacc gcctctgaac atcctgacac 481 catacagaga tgacaagaag gatgggctga agttctatac tgatccttcc tatttctttg 541 acctctggaa agaaaaaatg ctacaggaca cagaagacaa aaggaaagag aaaaggcgtc 601 aaaagagaga gaaacacaag ctgaacccta acagaaacca gcaagtaaat gtgagaaaag 661 tcagaacaag aaaagaagag tgggagagaa ggaaaatggg cattgagttt atgagtgacg 721 caaagaaact ggagcaggca gggagcgcca aagaggacag agtgcccagc gggtcacatg 781 catcggacgt tacggattac tcttacccgg ctactcccaa ccattctctg cacccccagc 841 ctgtgacccc ttcctatgca gctggtgacg tgccaccaca cgggcctgca agccaggctg 901 cggagcatga gtaccggccc ccatctgcct cggcgaggca catggccctc aacagacctc 961 agcagccgcc ccccccgcct ccccctcagg ccccagaggg gtcccaggcc tctgcaccga 1021 tggctccagc agactacggg atgctcccag cgcagataat tgagtattac aacccatccg 1081 gaccacctcc tccgccacct cctcctgtga ttccctcagc acaaactgcc ttcgtcagcc 1141 ctctccagat gcccatgcag cccccgttcc ctgcatcagc cagctccacg cacgcagctc 1201 ctcctcaccc accctccacc gggctcctgg tcacagcccc gccacccccg ggcccaccac 1261 ctcccccgcc aggccctcct ggtcccgggt cttcTCTTTC GTCCTCCCCA ATGCATGGCC 1321 CCCCAGTAGC TGAGGCGAAG CGGCAAGAGC CTGCACAGCC ACCAATCAGT GATGCTCGAA 1381 GCGACCTCCT CGCTGCTATT CGAATGGGAA TTCAACTGAA AAAGGTGCAG GAGCAGCGGG 1441 AGCAGGAGGC CAAGCGGGAG CCAGTGGGGA ATGACGTGGC CACGATCCTG TCCCGGCGCA 1501 TTGCCGTGGA GTACAGCGAC TCTGACGACG ACTCAGAGTT CGACGAGAAC GACTGGTCCG 1561 ACTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1621 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1681 GGCTTTATAT ATCTTGTGGA AAGGACGACG GTTCTGAACG AAAAGGTCGA TAACGCGTTA 1741 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt